小麦黄色花叶病毒不同分离物外壳蛋白(CP)基因序列的比较分析  

SEQUENCE COMPARISON OF THE GENE FOR COAT PROTEIN FROM DIFFERENT ISOLATES OF WHEAT YELLOW MOSAIC VIRUS

作　　者：韩成贵[1] 李大伟[1] 于嘉林[1] 邢怡明[1] 朱坤 田兆丰 蔡祝南[1] 刘仪[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室和植物病理学系,北京100094

出　　处：《植物病理学报》1999年第3期216-220,共5页Acta Phytopathologica Sinica

基　　金："863"计划课题;国家自然科学基金

摘　　要：核苷酸序列分析结果表明， 小麦黄色花叶病毒（Ｗ ＹＭＶ） 不同分离物的外壳蛋白基因存在一定的差异。邓州分离物ＣＰ基因在其３１～３３ｎｔ处均缺失了３个核苷酸，其余分离物与潢川分离物及日本分离物长度一致， 均为８８２ｎｔ。不同分离物ＣＰ基因核苷酸序列同源性为９７．３％ ～９８．９％ ， 由此推导的氨基酸序列同源性为９７．６％ ～９９．３％ ，外壳蛋白Ｎ 末端的１１０个氨基酸和 Ｃ末端的５５个氨基酸在各个分离物间是高度保守的。潢川分离物有５个氨基酸与其它５个分离物明显不同。Ｗ ＹＭ Ｖ 不同分离物外壳蛋白序列分析结果进一步确认了Ｗ ＹＭ Ｖ 与 ＷＳＳＭＶ 为 Ｂｙｍ ｏｖｉｒｕｓ 属的２种不同病毒。The sequence analysis revealed that there were some nucleotide differences in CP genes of wheat yellow mosaic virus (WYMV) from Dengzhou city in Henan, Yangzhou city in Jiangsu , Yaan city in Sichuan, Zhouzhi county in Shaanxi province of China. Comparing with the Huangchuan isolate and Japanese isolate, the CP gene of Dengzhou isolate had three nucleotides deletion (31-33nt) at its 5′end, the rest isolates had the same length CP gene,containing 882 nucleotides. The isolates of WYMV exhibited nucleotide sequence homologies ran ging from 97.3% to 98.9% and shared deduced amino acid sequence homologies of 97.6% to 99.3%,110 amino acid residues of the N terminal sequences and 55 amino acid residues of C terminal sequences in CP of different WYMV isolates were extremely conservative. Huangchuan isolate had 5 amino acid residues obviously different from the rest 5 isolates. These sequence data on WYMV CP gene from different isolates further indicate that WYMV and wheat spindle streak mosaic virus (WSSMV) are two distinct virus species within the genus Bymovirus .

关 键 词：小麦 黄色花叶病毒 分离物 外壳蛋白基因 

分 类 号：S435.121.9[农业科学—农业昆虫与害虫防治]