海南暗罗枝和叶中总三萜酸的含量测定  

Determination of the Content of Triterpenoidic Acid in Stems and Leaves of Polyalthia laui

作　　者：翟明君[1] 刘冰晶[1,2] 陈光英[1,2] 简蓝[1] 毕和平[1,2] 鲍长余[1,2] 孔杜林[1,2] 

机构地区：[1]海海南师范大学化学与化工学院,海南海口571158 [2]海南师范大学"省部共建-热带药用植物化学"教育部重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2010年第4期407-409,422,共4页Journal of Hainan Normal University（Natural Science）

基　　金：海南省高校硕士研究生创新科研课题(Hxwsy2009-10);国家大学生创新实验项目(101165815)

摘　　要：建立了测定海南暗罗枝和叶中总三萜酸含量的方法.以乌苏酸为对照品,以质量分数5%香草醛-冰醋酸和高氯酸溶液系统为显色剂,采用紫外分光光度法在546nm波长处测定样品的吸光度.乌苏酸对照品在0.20～1.60g·mL-1范围内呈良好线性关系,回归方程为Y=0.1548C+0.0286,r=0.9993.海南暗罗枝和叶中的总三萜酸含量分别为0.72%和1.56%.本法操作简便,结果可靠,重复性好,可用作海南暗罗中总三萜酸的含量测定.A method for the determination of the total triterpenoidic acid in the stems and leaves of P.laui was established.The total triterpenoidic acid in the stems and leaves of M.Paniculata Laui were determined by Ultraviolet Spectrophotometric method at 546 nm wavelength.The result showed a good linear relationship at the range of 0.20～1.60 μg·m L-1,and the curve was Y = 0.1548 C ＋0.0286,r = 0.9993.The content of stems was 0.72% and of leaves was 1.56%.This spectrophotometric method is simple,reproducible and suitable for the determination of total triterpenoidic acid.

关 键 词：海南暗罗 三萜酸 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]