紫外分光光度法测定斜脉暗罗叶和枝总三萜酸的含量  

Ultraviolet Spectrophotometric Determination of Tritepenoidic Acid in the Leaves and Stems of Polyalthia Plagioneura

作　　者：简蓝[1] 刘冰晶[1,2] 陈光英[1,2] 毕和平[1,2] 鲍长余[1,2] 翟明君[1] 

机构地区：[1]海海南师范大学化学与化工学院,海南海口571158 [2]海南师范大学"省部共建-热带药用植物化学"教育部重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2010年第4期410-412,448,共4页Journal of Hainan Normal University（Natural Science）

基　　金：海南省高校硕士研究生创新科研课题(Hxwsy2009-10);国家大学生创新实验项目(101165815)

摘　　要：测定斜脉暗罗叶和枝中总三萜酸的含量.以熊果酸为对照品,以质量分数5%香草醛-冰醋酸和高氯酸系统为显色剂,采用紫外分光光度法在546nm波长处测定样品的吸光度.熊果酸对照品在20～100g/mL范围内呈良好线性关系,回归方程为Abs=0.1548C+0.0286,r=0.9993.斜脉暗罗叶和枝中总三萜酸的含量分别为0.592%和1.436%.本法操作简便,结果稳定,重复性好,易于推广.Under the experimental conditions including ursolic acid as the reference,5% vanillin-acetic acid and perchloric acid as color system,the content of triterpenoidic acid in the leaves and stems of Polyalthia plagioneura was determined by ultraviolet spectrophotometric method at 546 nm.The calibration curve was linear（r = 0.999 3） in the range of 0.2～1.6μg /mL,and the curve was Abs = 0.154 8 C＋0.028 6,the content was 1.436% and 0.592%.The result was stable,and has good repeatability.This method was conveniently to operate and easy for application.

关 键 词：斜脉暗罗 三萜酸 紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]