T细胞免疫缺陷小鼠创伤性面瘫模型的建立及其形态学评价  

Establishment and morphological assessment of the T-cell immune deficiency mouse model with facial nerve axotomy

作　　者：全世明[1] 彭本刚[1] 高志强[2] 

机构地区：[1]北京积水潭医院耳鼻咽喉科,北京100035 [2]中国医学科学院中国协和医科大学北京协和医院耳鼻咽喉科,北京100730

出　　处：《山东大学耳鼻喉眼学报》2010年第6期5-9,共5页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：国家"十五"科技攻关项目(2004BA720A18-01);科技部科研院所社会公益研究专项基金(2002DB40097);教育部博士点专项基金(20060023017)资助项目

摘　　要：目的尝试建立T细胞免疫缺陷小鼠面瘫模型,并运用形态学技术分析免疫缺陷小鼠的面神经损伤特点,深入探讨面神经修复再生的神经免疫病理机制提供实验基础。方法切断裸鼠面神经出茎乳孔主干,于术后2周灌注固定动物,收集脑干切片,荧光金逆行示踪技术标记面运动神经元损伤情况,并结合面神经周围主干锇酸染色情况分析裸鼠面神经损伤特点。结果术后观察免疫缺陷小鼠面瘫出现情况,如瞬目反射、触须拂动、鼻尖方向、耳廓运动等均出现典型的完全周围性面瘫,术后14d裸鼠右侧面神经损伤远端锇酸染色显示面神经重度变性。面瘫小鼠面神经核团可见"健康"、"受损"、"死亡"的各型面运动神经元。T细胞免疫缺陷小鼠与野生型小鼠面神经核团计数有显著性差异。结论 T细胞免疫缺陷的小鼠创伤性面瘫模型稳定、可行,为进一步深入揭示外伤性面瘫发生与演进过程中以T细胞行为研究为中心的神经免疫病理机制提供了实验基础。Objective To establish a stable and reliable T-cell immune deficiency mouse model with facial nerve axotomy by the application and assessment with morphological methods on the immune deficiency mouse with facial nerve transection,so as to provide an experimental foundation for revealing the latent neuroimmunological mechanism of traumatic facial paralysis. Methods The T-cell deficiency mice were subjected to a right facial nerve axotomy. Fluorogold retrotracer was used at a designated time. Two weeks after the operation,the slices of the brain stem were collected and the facial moto-neurons were observed and counted. Also,the injured facial nerve of axotomy nude mice was assessed with osmic acid staining. Results All the mice presented typical facial paralysis after the transection of the facial nerve. Remarkable degenerations were observed in the distal end of the transected facial nerve on the 14th day after the operation. There was different status among the motoneurons in the facial nuclus,such as healthful,injured or dead. Significant difference in the number of facial moto-neurons between the nude mouse group and the wild type mouse group was noticed. Conclusion The T-cell immune deficiency mouse model with facial nerve axotomy is effectiveand feasible. It provides an experimental foundation for revealing the latent neuroimmunological mechanism centering on T cell behavior of the pathogenesis and progression of facial nerve trauma.

关 键 词：面神经 T细胞 免疫缺陷 动物模型 创伤 

分 类 号：R318.08[医药卫生—生物医学工程] R318.1[医药卫生—基础医学]