白介素-13对A431产生炎症及纤维化相关因子表达的影响  

Effect of Interleukin-13 on Inflammatory and Fibrosis-related Genes Expression in A431

作　　者：杨高云[1] 赵雅兰[2] 崔文颖[1] 陈雪[1] 孙雅纯[1] 徐薇[1] 乌日娜[2] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100095 [2]内蒙古医学院附属医院,呼和浩特010058

出　　处：《中国中西医结合皮肤性病学杂志》2010年第6期341-344,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金："教育部留学回国人员启动基金"支助

摘　　要：目的探讨白介素-13(IL-13)对皮肤鳞状细胞癌细胞系(A431)产生炎症及纤维化相关因子基因和蛋白表达的影响,从而进一步了解皮肤纤维化的发病机制。方法使用不同浓度的IL-13(0.1ng/mL、1ng/mL、10ng/mL)刺激体外培养的A431,24h后收集A431提取RNA、采用实时定量PCR(Real-Time PCR)法检测α-SMA、CTGF、pro-collagenⅢ、EMMPRIN和IL-13rα1的基因表达情况;同时收集细胞培养上清,应用ELISA方法检测A431经不同浓度IL-13刺激后TGF-β蛋白表达水平。结果 A431经不同浓度IL-13刺激24h后,其α-SMA、CTGF和pro-collagenⅢ的基因表达水平以及TGF-β蛋白表达水平明显高于对照组(P<0.05),并具有浓度依赖性。IL-13rα1和EMMPRIN基因表达与对照组无显著性差异(P>0.05)。结论 IL-13可刺激A431高表达α-SMA、CTGF和pro-collagenⅢ基因,并产生大量TGF-β,这些因子的高表达和大量产生可能参与了IL-13在皮肤纤维化过程中的病理性作用。Objective To investigate the effect of interleukin-13（IL-13） on inflammatory and fibrosis-related genes expression and protein production in A431.Methods Different concentrations of IL-13（0.1ng/mL,1ng/mL,10ng/mL）were used to stimulated the A431 that cultured in vitro.After 24 hours,ribonucleic acid（RNA） was extracted from A431.The Real-Time ploymerase chain reaction（PCR） assay was applied to measure the gene level of α-SMA,CTGF,IL-13rα1,EMMPRIN and Pro-collagen III in A431,and enzyme-linked immunosorbent assay（ELISA） was applied to measure the protein level of transforming growth factor（TGF）-β in the supernatant of A431.Results α-SMA,CTGF,pro-collagen III genes expression and TGF-β protein production were significantly and dose dependently increased in IL-13 treated A431（P〈0.05）,while IL-13rα1 and EMMPRIN genes expression were not significantly increased（P〈0.05）.Conclusion IL-13 could stimulate A431 over expressing genes of α-SMA,CTGF and Pro-collagen III,and over producing protein of TGF-β,these inflammatory and fibrosis related genes and protein may be involved in IL-13 pathological role in skin fibrosis.

关 键 词：白介素-13 A431 皮肤纤维化 基因表达 蛋白表达 

分 类 号：R593.25[医药卫生—内科学]