水稻条叶枯病毒云南分离物(RStV-Y)的纯化与理化特性分析  

Purification,Physical and Chemical Characterization of Yunnan Isolate of Rice Stripe Virus

作　　者：曲志才[1] 马向前[1] 曹清玉[1] 沈大棱[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《复旦学报（自然科学版）》1999年第4期447-450,共4页Journal of Fudan University：Natural Science

基　　金：美国麦氏(McKnight)基金;上海市自然科学基金

摘　　要：采用改进的水稻病毒纯化方法，以２００ ｇ／Ｌ的蔗糖垫替代蔗糖密度梯度，从ＲＳｔＶ 感染的水稻叶片中制备了病毒颗粒．电镜观察显示，ＲＳｔＶ 为细丝状和分枝状结构．变性琼脂糖凝胶电泳分析分离纯化的病毒总ＲＮＡ得到４ 个大小不同的病毒ＲＮＡ．ＳＤＳＰＡＧＥ可检测到病毒编码的外壳蛋白．提纯病毒作为抗原与所制备的抗血清及日本抗血清均有血清学反应．Rice stripe virus (RStV) from infected rice leaf was purified by a modified procedure of conventional purification with 200 g/L sucrose cushion instead of linear sucrose gradient. The virus with filamentous and branched filamentous particles were observed in purified preparations under electron microscope. Significant size differences were found in the single stranded forms of genomic RNAs of RStV by denatured agarose gel electrophoretic analysis. The viral coat protein was detected in SDS PAGE. With the antibody of RStV form Japan,the virus showed a serological homology. Thus,it indicates that this simple protocol is suitable for purifying the RNP particles of RStV.

关 键 词：水稻 条叶枯病毒 RStV 提纯 理化特性 病毒病 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]