玻璃化保存后老鼠黏附成骨细胞形态及存活率的实验观测  

Effect of cryoprotectants on the morphology and viability of attached murine osteoblast cells

作　　者：郝保同[1] 刘宝林[1] 王伯春[1] 戎森杰[1] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《上海理工大学学报》2010年第6期511-514,共4页Journal of University of Shanghai For Science and Technology

基　　金：国家自然科学基金项目(50776060);教育部新世纪优秀人才计划(07-0559);上海市重点学科建设资助项目(S30503)

摘　　要：利用玻璃化保存方法,在自行设计的细胞渗透性实验台上,实时观察了黏附成骨细胞在高浓度玻璃化保护液中的形态变化.经过七步添加和七步去除95%的VEG玻璃化保护液后,黏附的成骨细胞的形态由细长的纺锤形变为粗短的圆柱形,说明黏附细胞的两端及边缘的黏附点已脱落,但经过3~4 h的培养后,黏附细胞逐渐恢复了原来的形态,表明玻璃化保护液的处理只是改变了细胞的形态,没有造成黏附细胞的脱落和严重损伤.The morphology changes of attached osteoblast cells exposed to vitrification solutions of various concentrations were observed by use of a diffusion stage.After seven steps of sequential addition and removal of 95% VEG,the morphology of attaches cells changed from a long podium or spindle-like appearance to short cylinder shape.The cells appear to lose contact with the scaffold at the periphery of the cell,releasing focal adhesions and becoming more centralized.When incubated for 3~4 h,the attached cells recover and retain their original morphology.The results indicate that vitrification solution treatment changes only the morphology of attached cells,but does not damage the cells or cause detachment of cells.The results of the paper provide information for the cryopreservation of attached cells by vitrification.

关 键 词：玻璃化 低温保存 形态 黏附 

分 类 号：TB611[一般工业技术—制冷工程]