中国花生小核心种质SSR遗传多样性  

Genetic diversity of peanut mini core collection detected by SSR markers

作　　者：姜慧芳[1] 任小平[1] 张晓杰[1] 黄家权[1] 王圣玉[1] 雷永[1] 晏立英[1] 廖伯寿[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2010年第4期472-478,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家科技支撑计划(2006BAD13B05-2);国家自然科学基金(30571132);国家科技基础条件平台项目(2005DKA21002-13);农作物种质资源保护项目(NB05-070401-32)

摘　　要：从206对SSR引物中筛选26对引物对我国花生全套小核心种质298份资源进行了遗传多样性分析,相似系数为0.51～0.99,其中种质zhh1497与zhh1398遗传差异最大。检测到3个在不同植物学类型中特异表达的标记带型,包括普通型1个(2A06/440),龙生型1个(2A06/440),珍珠豆型1个(PM443/270),多粒型2个(PM443/270,9E08/500)。分析结果表明,多粒型花生的多态性信息量和遗传多样性指数均最大,平均相似系数最小,其次是普通型花生,龙生型和中间型花生的多态性信息量和遗传多样性指数均较小,平均相似系数较大。在我国花生小核心种质中,国外引入资源的多态性信息量和多样性指数均高于我国本土资源的对应值。在我国本土花生资源中,长江流域和南方地区资源的多态性信息量和遗传多样性指数均高于其他地区。A set of mini core collection comprised of 298 accessions was employed for SSR analysis.Twenty six polymorphic SSR markers were screened from a total of 206 primer pairs.The similarity coefficients between genotype pairs among the 298 accessions were 0.51 to 0.99.The genetic difference between the genotypes of zhh1497 and zhh1398 was the highest with similarity coefficient of 0.51 among these accessions.Three special SSR markers in botanical types were detected including 1 marker （2A06/440） in the type of hypogaea,1（2A06/440） in hirsuta,1 （PM443/270） in vulgaris and 2 （PM443/270,9E08/500） in fastigiata.Molecular polymorphism detected by SSR indicated that genetic diversity index were the highest in accessions of fastigiata,followed by hypogaea,vulgaris,hirsuta and then by the intermediate type.Among the Chinese mini core collection,both polymorphic information content and genetic diversity index were higher in introduced foreign accessions than the corresponding traits in the native accessions.In terms of Chinese native resource,the polymorphic information content and genetic diversity index were higher in accessions from Yangtze River valley and region of southern China than the corresponding traits in accessions from other regions of China.

关 键 词：花生小核心种质 SSR遗传多样性 多态性信息量 

分 类 号：S565.203[农业科学—作物学]