感染日本血吸虫小鼠用双氢青蒿素连续给药及与吡喹酮伍用的疗效观察  

Effect of multiple doses of dihydroarteminisin and combination with praziquantel against Schistosoma japonicum in experimental mice

作　　者：李洪军[1] 陶永辉[1] 戴建荣[1] 曲国立[1] 汪伟[1] 邢云天[1] 李幼子[1] 梁幼生[1] 

机构地区：[1]江苏省血吸虫病防治研究所、卫生部寄生虫病预防与控制技术重点实验室、江苏省寄生虫分子生物学重点实验室、江苏省寄生虫病学重点学科,无锡214064

出　　处：《中国血吸虫病防治杂志》2010年第6期534-538,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家科技支撑计划(2009BAI78B06);江苏省医学领军人才项目(LJ200608);江苏省卫生厅项目(X200707)

摘　　要：目的观察双氢青蒿素连续多次给药及与吡喹酮伍用对日本血吸虫童虫和成虫的杀灭效果。方法采用腹部贴片感染小鼠,每鼠感染日本血吸虫尾蚴(40±1)条后随机分组。分别在感染后第6天或第34天,用双氢青蒿素200、300、400mg/kg或600mg/kg灌服治疗,日1次,连服3d。在小鼠感染后第7天或第35天,分别灌服双氢青蒿素或吡喹酮各300mg/kg以及2种药物剂量同时灌服,或双氢青蒿素于小鼠感染后第7天或第35天灌服,而吡喹酮则在感染后第6天或第8天,以及第34天或第36天给药。2次试验各设有1组不治疗的对照。治疗组和对照组小鼠均于感染后50d解剖,收集成虫,计算减虫率和减雌率。结果在感染后第6天,连续3次灌服剂量分别为200、300、400mg/kg和600mg/kg双氢青蒿素,小鼠减虫率分别为69.16%、80.68%、87.11%和90.62%,减雌率分别为62.19%、75.61%、83.65%和92.16%。在感染后第34天,连续3次灌服给药组小鼠减虫率分别为73.90%、74.99%、84.19%和85.49%,减雌率分别为83.84%、92.91%、94.05%和95.27%。在童虫期(感染后7d),单剂量双氢青蒿素(300mg/kg)与吡喹酮(300mg/kg)联合给药组小鼠减虫率为19.66%;单剂量双氢青蒿素(300mg/kg)第7天服用,吡喹酮第6天或第8天给药组小鼠减虫率分别为42.96%和57.46%。在成虫期(感染后35d),双氢青蒿素(300mg/kg)与吡喹酮(300mg/kg)联合给药组小鼠减虫率为70.21%,吡喹酮第34天或第36天给药组小鼠减虫率分别为60.82%和81.51%。结论双氢青蒿素在童虫期和成虫期连续给药可增强其抗日本血吸虫作用效果。在童虫期,双氢青蒿素与吡喹酮伍用或吡喹酮第6天用药可降低抗日本血吸虫作用效果;在成虫期,双氢青蒿素与吡喹酮伍用或吡喹酮第36天用药可增强抗日本血吸虫效果。Objective To investigate the antischistosomal effect of multiple doses of dihydroarteminisin and combination with praziquantel against schistosomula and adults of Schistosoma japonicum.Methods Mice were infected with（40±1）S.japonicum cercaria on the abdomen,and then grouped randomly.On the 6th,7th and 8th and 34th,35th and 36th post-infection,mice were given continuously dihydroarteminisin at doses of 200,300,400 and 600 mg/kg daily,respectively.On the day 7th or 35th,mice were given 300 mg/kg dihydroarteminisin combined with 300 mg/kg praziquantel on the day 6th or 8th,and 300 mg/kg dihydroarteminisin plus 300 mg/kg praziquantel on the day 34th or 36th.All mice were sacrificed 50 d post-infection,the adult worms were collected,and the worm reduction rates and female worm reduction rates were calculated.Results On the 6th to 8th day post-infection,the worm reduction rates of mice were 69.16%,80.68%,87.11%and 90.62%,respectively,and the corresponding female worm reduction rates were 62.19%,75.61%,83.65% and 92.16%,respectively,after given dihydroarteminisin at doses of 200,300,400 and 600 mg/kg for 3 continuous times.On the 34th to 36th day post-infection,after 3 continuous times of administration,the worm reduction rates were 73.90%,74.99%,84.19% and 85.49%,respectively,with corresponding female worm reduction rates of 83.84%,92.91%,94.05% and 95.27%,respectively.During the stage of schistosomulum（7 d post-infection）,the worm reduction rate was 18.66% after given a combination of 300 mg/kg dihydroarteminisin and 300 mg/kg praziquantel.After administration of a single dose of 300 mg/kg dihydroarteminisin on the 7th day,plus praziquantel on the 6th day or8th day,the worm reduction rates were 42.96% and 57.46%,respectively.During the stage of adults（35 d post-infection）,the worm reduction rate was 70.21% after given a combination of 300 mg/kg dihydroarteminisin and 300 mg/kg praziquantel.After administration of a single dose of 300 mg/kg dihydroarteminisin,plus praziquantel on the 34th day or 36th day,t

关 键 词：日本血吸虫 双氢青蒿素 吡喹酮 杀虫效果 联合用药 

分 类 号：R532.21[医药卫生—内科学]