管式磁性微粒子化学发光免疫分析法测定人尿液中的雌三醇  

Magnetic Microparticle Chemiluminescence Immunoassay Method for Determination of Estriol in Human Urine

作　　者：郑国金[1,2] 方卢秋[3,2] 陈惠[2] 李振甲[4] 应希堂 林金明[2] 

机构地区：[1]楚雄医药高等专科学校化学教研室,楚雄675000 [2]清华大学化学系,北京100084 [3]长江师范学院化学化工学院,重庆408100 [4]北京科美东雅生物技术有限公司,北京100094

出　　处：《分析化学》2011年第1期62-66,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.90813015)资助项目

摘　　要：待测尿液中的雌三醇、辣根过氧化物酶标记的雌三醇与异硫氰酸荧光素(FITC)标记的兔抗雌三醇抗体在均相体系中发生竞争性免疫反应,再加入用羊抗FITC抗体包被的磁微粒,反应生成物结合在磁微粒上,在磁场中经分离、洗涤后加发光底物,检测发光强度(RLU),测定尿液中雌三醇的含量。通过对检测条件的优化,建立了磁性微粒子化学发光免疫分析法测定人尿液中雌三醇的方法,并对正常男性、女性和孕妇的尿液中雌三醇含量进行了测定。结果表明,本方法的线性范围为1～100μg/L,检出限为0.25μg/L,具有很高的灵敏度;批内相对标准偏差<14%;批间相对标准偏差<7%,具有良好的稳定性和重现性。A magnetic microparticle chemiluminescence immunoassay method was developed for the determination of estriol in human urine.The sample of estriol and horseradish peroxidase（HRP） labeled estriol with fluorescein isothiocyanate（FITC） labeled rabbit anti-estriol antibodies were mixed in homogeneous system.The reaction product in homogeneous system can be combined onto the goat anti-FITC antibody-coated magnetic particles.After the magnetic field separation of bond and free,the light-emitting substrate was added to magnetic particles.Under the optimization conditions,this methods can be used to detect estriol in a linear range of 1-100 μg/L,detection limit of 0.25 μg/L,relative standard deviation less than 14% and betweenrelative standard deviation less than 7%.The method has been applied for the determination of estriol in real samples,and the results are satisfactory.

关 键 词：雌三醇 化学发光免疫分析 磁性微粒子 尿液 

分 类 号：R446.6[医药卫生—诊断学]