辛巴蓝F-3GA修饰的啤酒废酵母菌亲和吸附溶菌酶  

Adsorption of Waste Beer Yeast Modified with Cibacron Blue F-3GA for Lysozyme

作　　者：陈聪[1] 孙小梅[1] 李步海[1] 

机构地区：[1]中南民族大学化学与材料科学学院,分析化学国家民委重点实验室,武汉430074

出　　处：《分析化学》2011年第1期91-94,共4页Chinese Journal of Analytical Chemistry

基　　金：国家民委院校科研基金(No.MJY00004)资助项目

摘　　要：利用三嗪染料辛巴蓝F-3GA修饰经戊二醛交联的啤酒废酵母菌,得到一种新型染料亲和吸附剂。辛巴蓝F-3GA的固载量为161.1mg/g。以溶菌酶为研究对象,考察吸附时间、酶初始浓度、pH值、离子强度等因素对吸附率的影响。结果表明:当pH=7.0时,其对溶菌酶有较高的吸附量(229.1mg/g),吸附性能明显优于未接枝F-3GA的交联菌。负载的溶菌酶用1.0mol/LNaSCN溶液洗脱,洗脱率高达89.1%,酶活性保持率为91.0%。将其用于鸡蛋清中溶菌酶的分离纯化,酶活力收得率为73.5%,纯化倍数为26.7。The waste beer yeast was crosslinked with glutaraldehyde and modified with Cibacron B1ue F-3GA.A new dye affinity adsorbent was prepared.The amount of immobilized Cibacron Blue F-3GA on the crosslinked yeasts was 161.1 mg/g.The effects of contact time,initial lysozyme concentration,medium pH and ionic strength on the adsorption of modified yeasts for lysozyme were studied.The dye affinity adsorbents were used for lysozyme purification from chicken egg white.The lysozyme adsorption capacity of the modified yeast was 229.1 mg/g,its adsorption performance was better than the unmodified yeast.The high desorption rate（89.1%） was achieved by using 1.0 mol/L NaSCN as eluate,the rate of activity-preserving was 91.0%.In extracting lysozyme from chicken egg white,the purification fold and average recovery yield of lysozyme activity were 26.7% and 73.5%,respectively.The adsorbents could be reused without significant decreases in the adsorption capacities.

关 键 词：啤酒废酵母菌 辛巴蓝F-3GA 染料亲和吸附 溶菌酶 

分 类 号：TQ320.721[化学工程—合成树脂塑料工业]