镧掺杂二氧化钛/沸石微柱在线预富集-分光光度法测定溶菌酶含量  

Spectrophotometric Determination of Lysozyme by On-line Preconcentration with A Microcolumn Containing La^(3+)-TiO_2-Zeolite

作　　者：田苗苗[1] 苏日艳[1] 贾琼[1] 鲍长利[1] 权新军[1] 

机构地区：[1]吉林大学化学学院,长春130022

出　　处：《分析化学》2011年第1期103-106,共4页Chinese Journal of Analytical Chemistry

基　　金：中国科学院长春应用化学研究所稀土资源利用国家重点实验室开放基金(2008)项目

摘　　要：以镧掺杂二氧化钛/沸石吸附剂微柱为萃取装置,建立了溶菌酶的分光光度检测法。考察了实验条件对萃取效率的影响。以甲醇为洗脱液,流速2.0mL/min;样品的pH=5.0,流速1.2mL/min,体积1.2mL;检测波长280nm。对蛋清、蜂蜜及葡萄酒中溶菌酶的含量进行了测定,日内与日间精密度分别为2.1%和2.8%;加标回收率>90%。结果表明,建立的镧掺杂二氧化钛/沸石微柱在线预富集-分光光度法具备简单、快速、灵敏度高等优点,适合于蛋清、蜂蜜及葡萄酒中溶菌酶的测定。A spectrophotometric method for the determination of lysozyme was established based on the on-line preconcentration with a La3 ＋-TiO2-zeolite microcolumn.Effects of operation parameters on the extraction efficiency were optimized as follows：methanol as the eluent with a flow rate of 2.0 mL/min,sample pH of 5.0,sample flow rate of 1.2 mL/min,sample volume of 1.2 mL,and detection wavelength of 280 nm.The proposed method was employed for the determination of lysozyme in egg white,honey,and wine samples.The relative standard deviations of intra-day and inter-day were determined as 2.1% and 2.8%,respectively.The recoveries were higher than 90%.The method is simple,rapid,sensitive.

关 键 词：镧掺杂二氧化钛/沸石 微柱 分光光度法 溶菌酶 

分 类 号：O658.9[理学—分析化学]