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机构地区:[1]成都中医药大学,成都611137 [2]西南科技大学,四川绵阳621010
出 处:《中国实验方剂学杂志》2011年第2期1-5,共5页Chinese Journal of Experimental Traditional Medical Formulae
基 金:国家自然科学基金(30801550)
摘 要:目的:研究当归补血汤提取液的相状态。方法:通过HPLC检测阿魏酸、HPLC-ELSD检测黄芪甲苷、紫外分光光度法检测总多糖、双缩脲法检测蛋白质,并以激光衍射法检测粒径和粒径分布,结合光学现象,描述当归补血汤的相状态。结果:可以测得当归补血汤中阿魏酸、黄芪甲苷、总多糖和蛋白质的含量,提取液中分散相质点的粒径主要分布于50~100 nm和1 000 nm以上,并可以观察到明显的丁达尔现象。结论:当归补血汤含有以均相状态存在的真溶液和非均相状态存在的胶体溶液、混悬液或乳浊液,为混合分散体系。Objective:To study the phase state of Danggui Buxue decoction.Method:The phase state of Danggui Buxue decoction was described by means of HPLC(for ferulic acid),HPLC-ELSD(for astragaloside Ⅳ),UV spectrophotometry(for total polysaccharides),biuret method(for total protein) and laser diffraction technique(for particle size and particle size distribution),in combination with optical phenomena.Result:In Danggui Buxue decoction,ferulic acid,astragaloside Ⅳ,total polysaccharide and total protein could be detected,particle size distribution was in the range of 50-100 nm,1 000 nm and above,and Tyndall phenomenon could be observed clearly.Conclusion:The phase state of Danggui Buxue decoction was composed of the true solution in homogeneous state,and colloidal solution,suspension or emulsion in heterogeneous state.Danggui Buxue decoction was a disperse system.
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