刺五加中紫丁香苷、刺五加苷E的大孔树脂分离纯化工艺  

Separation and Purification of Syringin and Elentheroside E by Macroporous Adsorbents

作　　者：邵佳锋[1] 刘树民[1] 牟洪[1] 范振群[1] 安丽凤[1] 董杨[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2011年第2期10-14,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103)

摘　　要：目的:研究大孔树脂分离纯化紫丁香苷、刺五加苷E的工艺。方法:采用AB-8型大孔吸附树脂对紫丁香苷、刺五加苷E进行吸附纯化,并采用HPLC,以紫丁香苷、刺五加苷E含量和收率为参考指标综合评价。结果:优选纯化工艺为,上样液的质量浓度0.5 g·mL-1,吸附流速2 BV·h-1,洗脱剂30%乙醇,用量9 BV,洗脱流速1 BV·h-1。结论:刺五加提取物通过AB-8树脂,30%乙醇洗脱部位,可使紫丁香苷、刺五加苷E的收率和质量分数达到满意效果。Objective：To establish a technical process for the purification of syringin and eleutheroside E from Acanthopanax senticosus.Method：AB-8 type of macroporous adsorbents was used to separate and purify syringin and eleutheroside E,and HPLC was adopted to analyze the content and yield of syringin and eleutheroside E.Result：The optimum process for the purification was as follows：the concentration of sample solution was 0.5 g·mL-1;adsorption flow rate was 2 BV·h-1;eluent was 30% ethanol;the dosage was 9 BV;elution flow rate was 1 BV·h-1.Conclusion：Extract of A.senticosus by AB-8 resin,30% ethanol elution positions can syringin,eleutheroside E,yield and quality of the scores achieved satisfactory results.

关 键 词：大孔吸附树脂 分离 纯化 紫丁香苷 刺五加苷E 

分 类 号：R283.6[医药卫生—中药学]