银屑灵片中芍药苷和甘草酸的提取工艺  

Extract Process Optimization of Peoniflorin and Glycyrrhizic Acid in Yinxieling Tablet by Orthogonal Design

作　　者：冯丽敏[1] 赵瑞芝[1] 王银洁[1] 韩凌[1] 卢传坚[1] 

机构地区：[1]广州中医药大学第二临床医学院中药制剂实验室,广州510006

出　　处：《中国实验方剂学杂志》2011年第2期25-27,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金(7004847;10251040701000001);广东省科技厅项目(2010A03010008);广州市科技开发项目(2010J-E031);广东省中医药科学院中医特色与优势临床专项(2010年第35号)

摘　　要：目的:建立高效液相色谱同时测定银屑灵片中芍药苷和甘草酸含量的方法,采用正交设计法优选银屑灵片中芍药及甘草有效成分的提取工艺。方法:以芍药苷和甘草酸为指标,采用正交试验,考察乙醇浓度、提取时间、提取次数等因素的影响,优化提取条件。结果:最佳提取工艺条件为:70%乙醇提取2次、每次提取0.5 h。结论:优选的提取工艺多种有效成分的提取率均较高,适用于生产。Objective：Optimizing the extract process for Yinxieling tablet.Method：The content of peoniflorin and glycyrrhizic acid in the extract was used as the index,and the concentration was determined by HPLC.The concentration of alcohol,extract time and number of times were considered.The orthogonal test was used for optimization.Result：The optimum extract process was as follows：70% alcohol extracted for 0.5 h,the extract number of times was twice.Conclusion：The optimum process provided a high yield for both three components,and easy to carry out.

关 键 词：银屑灵片 芍药苷 甘草酸 正交试验 提取工艺 

分 类 号：R283.6[医药卫生—中药学]