连理汤中人参皂苷Rb_1含量测定  

Determination of Ginsenoside Rb_1 in Lianli Tang by HPLC Method

作　　者：王淳[1] 宋志前[1] 李青[1] 夏磊[1] 王超[1] 刘振丽[1] 

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第2期68-69,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院自主选题团队建设项目(Z02028)

摘　　要：目的:建立高效液相色谱法(HPLC)测定连理汤中人参皂苷Rb1含量的方法。方法:采用Zobax Extend-C18(4.6 mm×150 mm,5μm)色谱柱,流动相乙腈-水(25∶75);检测波长203 nm;流速1.0 mL·min-1;柱温40℃。结果:两个批次连理汤样品中人参皂苷Rb1含量分别为1.58 mg.g-1和1.47 mg·g-1。结论:方法稳定可靠,重复性好,可用于连理汤中人参皂苷Rb1的含量测定并控制制剂的质量。Objective：To develop a HPLC method for the determination of Ginsenoside Rb1 in Lianli Tang.Method：The HPLC method was applied with Agilent Zobax Extend-C18 colunmn by acetonitrile-water（25∶75） as the mobile phase,The flow rate was 1.0 mL·min-1.The column temperature was 40℃ and the UV detection wavelength was 203 nm.Result：The contents of ginsenoside Rb1 in two batch samples are 1.58 mg·g-1 and 1.47 mg·g-1.Conclusion：The method is stable and reliable,and can be used to assay ginsenoside Rb1 then control the quality of Lianli tang.

关 键 词：连理汤 人参皂苷RB1 含量 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]