HPLC-ELSD联用检测白头翁汤中白头翁皂苷B_4的含量  

Determination of Pulchinenoside B_4 in Baitouweng Decoction by HPLC-ELSD

作　　者：刘丽梅[1] 柏冬[1] 王瑞海[1] 

机构地区：[1]中国中医科学院基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第2期70-72,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院自主选题研究项目课题(Z02028)

摘　　要：目的:建立HPLC-ELSD检测白头翁汤中白头翁皂苷B4含量的方法。方法:采用Agilent Ecilpse XDB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(28∶72)为流动相;ELSD漂移管温度100℃,雾化气体(空气)流速2.8 L·min-1。结果:白头翁皂苷B4在1.156～10.404μg线性关系良好,回收率为98.9%(n=5),RSD 0.7%。结论:本法准确、灵敏、重复性好,为白头翁汤的质量控制提供了依据。Objective：To establish a method for the deter mination of pulchinenoside B4 in Baitouweng decoction by HPLC-ELSD.Method： A HPLC methods detected by HPLC-ELSD has been developed： Agilent Ecilpse XDB-C18 column（4.6 mm × 250 mm,5 μm） with mobile phase of acetonitrile and water（28∶72）;The temperature of the drift tube was set at 100 ℃,the flow rate of gas at 2.8 L·min-1.Result：pulchinenoside B4 in Baitouweng decoction could be deter mined by HPLC-ELSD.The line arrange of pulchinenoside B4 was 1.156-10.404 μg.The recovery was 98.9%（n = 5,RSD 0.7%）.Conclusion：The method is simple and accurate,with good repeatability,and can be used for deter mination of pulchinenoside B4 in Baitouweng decoction.

关 键 词：白头翁汤 白头翁皂苷B4 含量测定 

分 类 号：R284.1[医药卫生—中药学]