苦豆子总碱对S180荷瘤小鼠的抑瘤作用  

Effects of Total Alkaloid of Sophora alopecuroides on S180 Tumor Mice

作　　者：焦河玲[1] 邓虹珠[2] 王晓娟[2] 刁建新[2] 原海晓[2] 姚辉[2] 

机构地区：[1]南阳理工学院,河南南阳473000 [2]南方医科大学中医药学院,广州510515

出　　处：《中国实验方剂学杂志》2011年第2期163-165,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广州市科技计划项目(2006Z2-E5053)

摘　　要：目的:观察苦豆子总碱(total alkaloid of Sophora alopecuroides,TASA)对S180荷瘤小鼠的抑瘤作用及其对小鼠血清肿瘤坏死因子(TNF-α)和白介素-6(IL-6)的影响。方法:建立小鼠移植性S180肉瘤模型。随机分为空白对照组、荷瘤对照组、环磷酰胺(CTX)组、苦豆子总碱高、中、低剂量(分别为80,40,20 mg.kg-1)组。连续给药10 d,观察小鼠一般情况;10 d后取血,处死小鼠,分离瘤块,计算抑瘤率;酶联免疫吸附法(ELISA)检测小鼠血清TNF-α,IL-6的水平。结果:TASA高剂量组肿瘤生长受到抑制(P<0.05);TASA各剂量组TNF-α含量与荷瘤对照组相比显著升高(P<0.05);TASA的高剂量组、中剂量组IL-6含量比荷瘤对照组显著升高(P<0.05)。结论:苦豆子总碱有抗肿瘤活性,其作用可能与提高荷瘤小鼠血清TNF-α,IL-6含量有关。Objective：To investigate the effects of total alkaloid of Sophora alopecuroides（TASA） on serum tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） expressions and therapeutic effects in S180 tumor-bearing mice.Method：The model of S180 tumor-bearing mice was established.The animals were divided into control group,tumor control group,cyclophosphamide（CTX） group,TASA high,middle and low dose groups（80,40,20 mg·kg-1）.After administration of TASA for 10 days,the general conditions of mice were observed.The rate of inhibition of tumor was detected.The levels of TNF-α and IL-6 in serum were measured by ELISA.Result：The contents of TNF-α and IL-6 in TASA group were higher than that in tumor-bearing control group（P 0.05）.Conclusion：TASA can inhibit tumor growth in vivo by increasing the activity of TNF-α and IL-6.

关 键 词：苦豆子总碱 荷瘤小鼠 抑瘤率 肿瘤坏死因子-α 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]