紫杉醇对人口腔鳞癌KB细胞生长抑制作用及诱导凋亡作用的研究  

Effects of Paclitaxel on Growth Inhibition and Pro-apoptosis of Human Carcinoma of Mouth Floor KB Cells

作　　者：赵蓉[1] 贾荣娣[1] 汪华君[1] 

机构地区：[1]江苏大学附属医院,江苏镇江212001

出　　处：《中国实验方剂学杂志》2011年第2期177-180,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究紫杉醇对人口腔鳞癌KB细胞生长抑制及凋亡的作用。方法:MTT方法检测紫杉醇对人口腔鳞癌KB细胞生长抑制率,计算IC50;将0.25,0.5,1μmol·L-1的紫杉醇作用于KB细胞48 h,分别用DAPI染色和流式细胞术检测细胞凋亡,并用免疫印迹法检测紫杉醇对凋亡相关蛋白Bax,Bcl-2和pro-caspase-3的影响。结果:紫杉醇对KB细胞的生长有明显的抑制作用,DAPI染色可见典型的凋亡细胞形态学特征,流式细胞术检测结果表明紫杉醇增加KB细胞凋亡率,免疫印迹结果显示紫杉醇可以增加Bax表达,减少Bcl-2表达,激活caspase-3。结论:紫杉醇对人口腔鳞癌KB细胞有生长抑制和诱导凋亡作用,这可能与抗癌机制有关。Objective：To explore the effects of paclitaxel on the growth and apoptosis of human carcinoma of mouth floor KB cells.Method： Growth inhibitory effect of paclitaxel on KB cells was detected by MTT assay and IC50 value was calculated.Human carcinoma of mouth floor KB cells were cultured with 0.25,0.5 and 1 μmol·L-1 paclitaxel for 48 h.The cell apoptosis was detected by DAPI staining and flow cytometry.Western blot was used to explore the effects of paclitaxel on apoptosis-regulated proteins,such as Bax,Bcl-2 and pro-caspase-3.Result： Paclitaxel inhibited the growth of KB cells.A typical feature of apoptosis was observed by DAPI staining.The flow cytometry showed that the apoptosis rate increased after paclitaxel treatment.Western blot showed that the expression of Bcl-2 decreased and Bax increased,and caspase-3 was activated.Conclusion： Paclitaxel can inhibit the growth of human carcinoma of mouth floor KB cells and induce its apoptosis,which might be one of anti-cancer mechanisms.

关 键 词：紫杉醇 人口腔鳞癌KB细胞 生长抑制 凋亡 

分 类 号：R285.5[医药卫生—中药学]