柴胡总皂苷醇洗脱精制品对大鼠长期毒性研究  

Study on Chronic Toxicity of Refined Products of Bupleurum Saikosaponin with Alcohol Elution in Rats

作　　者：黄伟[1,2] 刘芬娣[3] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,济南250014 [2]山东中医药大学,济南250355 [3]北京语言大学医院,北京100083

出　　处：《中国实验方剂学杂志》2011年第2期213-218,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30672649);山东省科技平台建设项目(2008GG2NS02021);山东省科技公关关键技术研究课题(2007GG2NS02073);山东省国际合作引智项目(L20083700336);国家863科技攻关计划课题(2004BA721A15);国家重点基础研究发展计划(973)项目(2009CB522802)

摘　　要：目的:连续观察45 d给大鼠柴胡总皂苷醇洗脱精制样品导致大鼠长期毒性损伤程度及恢复情况。方法:分别给大鼠灌胃高、中、低剂量的柴胡总皂苷醇洗脱精制样品连续45 d,按柴胡总皂苷计算,高、中、低剂量组分别为60,30,15 mg·kg-1,除观察一般状况外,检测血常规,血清肝功、肾功、脂质代谢、糖代谢相关指标;剖杀大鼠,精密称取心、肝、脾、肺、肾脏质量,计算脏体比值;进行肝脏病理组织学检查。剩余大鼠停止给药,恢复30 d,观察大鼠恢复情况,并进行上述相同指标的检测。结果:不同剂量的柴胡总皂苷醇洗脱精制样品在给药后45 d可导致大鼠体重下降,导致血中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBI)、总蛋白(TPC)、尿素氮(BUN)水平明显增高,白蛋白(ALB)明显降低,导致肝脏质量和肝体比值增大,病理学检查可见不同程度的肝组织损伤;上述变化随剂量的增加而逐渐加重,与空白组比较有明显差异。对碱性磷酸酶(AKP)、血糖(Glu)、胆固醇(CHO)、肌酐(Cr)等指标和血常规影响不明显。恢复期大鼠上述各指标与空白组比较均无显著性差异。结论:长时间给予一定剂量的柴胡总皂苷醇洗脱精制品可造成对大鼠明显的蓄积毒性,其毒性损伤部位以肝、肾损伤为主,经过30 d恢复期观察,其脏器损伤基本可恢复。Objective： To observe the chronic toxicity damage of the refined products of bupleurum saikosaponin with alcohol elution to rats who were continuously administrated for 45 days and its recovery condition.Method：Separately lavage different dose refined products of Bupleurum saikosaponin with alcohol elution to rats,and the high,middle and low lose-group are separately 60,30,15 mg·kg-1 caculated by total saikosaponin,the general conditions were observed and the related indexes were detected,such as the routine control of blood,hepatic function,renal function,the metabolism condition of lipids and the glycometabolism indexes;the rats were dissected and precisionly weigh the weight of heart,liver,spleen,lung,renal,and calculated the ratio of organ to body;thehistopathologic examination of hepatic tissue were examined.The remaining rats were stopped administrating,and recovered for 30 days,observed the recovery condition of rats,and the above indexes were detected.Result：Different dose refined products of Bupleurum saikosaponin with alcohol elution could induce the rats＇ body weight decreasing,the level of alanine aminotransterase（ALT）,aspartate aminotransterase（AST）,total bilirubin（TBI）,total protein（TPC）,and blood urea nitrogen（BUN） in serum obviously increasing,alburnin（ALB） in serum obviously decreasing,the liver weight and the ratio of liver to body increasing,and the hepatic tissue damage was obvious in the histopathologic examination of hepatic tissue;the above-mentioned change gradually aggravated with dose increasing,and it was the obvious discrepancy compared with control group.The influence on the content of alkaline phosphatase（AKP）,glucose（Glu）,cholesterol（CHO） and Cr was not obvious.The above indexes of recovered rats had no obvious discrepancy compared with control group.Conclusion：The long term and certain dose administration of refined products of Bupleurum saikosaponin with alcohol elution to rats could induce obvious cumulative toxicity,and the mainly t

关 键 词：柴胡总皂苷醇洗脱精制品 大鼠 长期毒性 

分 类 号：R285.5[医药卫生—中药学]