RP-HPLC法测定人血清中维生素C的含量  

RP-HPLC determination of vitamin C in human serum

作　　者：刁娟娟[1] 孟磊[1] 孙炜[2] 赵效国[2] 勉强辉[1] 米合拉依.卡德尔 李新霞[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830054 [2]新疆医科大学公共卫生学院,乌鲁木齐830054

出　　处：《药物分析杂志》2011年第1期55-58,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立反相高效液相色谱法测定人血清中维生素C含量。方法：采用SHIMADZU VP-ODS色谱柱（4.6 mm×250mm,5μm）,流动相为30 mmol.L-1磷酸二氢钾溶液-甲醇（88∶12,pH3.0）,流速0.8 mL.min-1,检测波长243 nm,进样量20μL,外标法定量。结果：维生素C与人血清中其他成分分离良好。维生素C浓度在0.5~15.0 mg.L-1范围内呈良好的线性关系,r=0.9999。高、中、低3个浓度的日内RSD分别为0.5%,5.0%,2.1%;日间RSD分别为5.0%,6.5%,4.8%;平均回收率分别为93%,88%,84%。结论：采用反相高效液相色谱法测定人血清中维生素C的含量,样品处理简便,结果准确可靠。该方法可用于大量血清样品中维生素C的含量测定。Objective：To establish an RP-HPLC method for the determination of vitamin C in human serum.Methods：The chromatographic column was SHIMADZU VP-ODS（4.6 mm×250 mm,5 μm）,the mobile phase was 30 mmol·L-1KH2PO4 solution-methanol（88∶12,pH 3.0） at the flow rate of 0.8 mL·min-1,detection wavelength was 243 nm,and injection volume was 20 μL,using external standard method.Results：Vitamin C was separated well from other ingredients in serum.The linear range was 0.5-15.0 mg·L-1（r=0.9999） for vitamin C.The intra-day RSDs of high,medium and low concentration were 0.5%,5.0%,2.1%,the inter-day RSDs were 5.0%,6.5%,4.8%,and the average recoveries were 93%,88%,84%,respectively.Conclusion：The RP-HPLC method proposed for the determination of vitamin C in human serum is simple,reliable,accurate,and can be applied for the determination of a great number of samples.

关 键 词：维生素C 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]