抗氧化活性藻蓝色素与牛血清白蛋白的相互作用  

Interaction between antioxidant phycocyanobilin and bovine serum albumin

作　　者：陈英杰[1,2] 刘少芳[1,2] 陈华新[1] 李富起[1] 秦松[1] 

机构地区：[1]中国科学院海洋研究所,实验海洋生物学重点实验室,青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《药物分析杂志》2011年第1期87-89,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家海洋863重点项目(2006AA090303);中科院知识创新工程重要方向性项目(KZCX2-YW-209,KZCX2-YW-216);山东省基金项目(ZR2009EQ003)

摘　　要：目的:进一步验证藻蓝色素(phycocyanobilin,PCB)的抗氧化作用,并研究藻蓝色素与牛血清白蛋白(bovine serum albu-min,BSA)之间的相互结合反应。方法:通过1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)清除法验证PCB的抗氧化作用。用荧光光谱法、分光光度法研究了PCB与BSA的相互结合反应。结果:PCB对DPPH自由基的清除作用呈现一定的量效关系。随着PCB浓度的增加,BSA的荧光强度有规律地降低且呈良好的线性关系。结论:抗氧化活性PCB能够有效地淬灭BSA的内源荧光,该猝灭作用属于静态荧光猝灭作用,反应的结合常数K=1.22×106L.mol-1,结合位点数n=1.14,与PCB的结合基本不会引起BSA的构象变化。Objective：To confirm the antioxidant activity of phycocyanobilin（PCB） and study the interaction between PCB and bovine serum albumin（BSA） in aqueous solution.Methods：The antioxidant of PCB was evaluated with DPPH method.And the interaction between PCB and BSA was measured by fluorescence and UV-Vis absorption spectrometries.Results：DPPH radicals scavenging activity of PCB related to its concentration.The fluorescence intensity of BSA decreased with the increasing of the concentration of PCB regularly,which had favorable linear relationship.Conclusion：PCB,with antioxidant activity,could effectively quench the fluorescence intensity of BSA through a single static quenching process.The binding constant K was 1.22×106 L·mol-1.The number of binding sites n was 1.14.Generally,the interaction with PCB did not change the conformation of BSA.

关 键 词：藻蓝色素(PCB) 牛血清白蛋白(BSA) 荧光淬灭 

分 类 号：R917[医药卫生—药物分析学]