HPLC法同时测定苦豆子中野靛碱和槐胺碱的含量  

HPLC simultaneous determination of cytisine and sophoridine in Sophora alopecuroides L.

作　　者：王俊卿[1,2] 马玲 刘利军[1] 王英华 

机构地区：[1]宁夏大学化工学院,银川750021 [2]宁夏回族自治区中医研究院,银川750021 [3]宁夏回族自治区药品检验所,银川750001

出　　处：《药物分析杂志》2011年第1期148-150,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：宁夏“十一五”科技攻关项目(宁科计字【2006】74号)

摘　　要：目的：建立HPLC法测定苦豆子中野靛碱和槐胺碱的含量。方法：采用Waters x-Bridge C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈-含2.0 mL.L-1三乙胺的0.05 mol.L-1的磷酸二氢钾溶液（90∶10）,流速为1.0 mL.min-1,检测波长为311 nm。结果：野靛碱和槐胺碱的线性范围分别为4.972~165.7μg（r=0.9994）和4.388~146.3μg（r=0.9991）;平均回收率（n=9）分别为101.8%和99.3%。结论：本方法简便、可靠、准确,可为苦豆子药材的开发利用提供理论依据。Objective：To develop an HPLC method for determination of cytisine and sophoridine in Sophora alopecuroides.Methods：Waters x-Bridge C18（4.6 mm×250 mm,5 μm） column was adoped;The mobile phase consisted of acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution containing 2.0 mL·L-1 triethylamine（90∶10） at the flow rate of 1 mL·min-1.The detection wavelength was set at 311 nm.Results：The linear ranges of cytisine and sophoridine were 4.972-165.7 μg（r=0.9994） and 4.388-146.3 μg（r=0.9991）;The recoveries of them were 101.8% and 99.3%.Conclusion：The method is simple,reliable,accurate,and can provide basis for development and utilization of Sophora alopecuroides.

关 键 词：高效液相色谱 苦豆子 野靛碱 槐胺碱 

分 类 号：R917[医药卫生—药物分析学]