妊娠期添加叶酸对新生仔猪肝脏抗氧化能力和基因表达影响  

Effects of Maternal Folic Acid Supplementation on Antioxidant Abilities and Gene Expression in Liver of Newborn Piglets

作　　者：刘静波[1] 陈代文[1] 余冰[1] 姚英[1] 

机构地区：[1]四川农业大学动物营养研究所,动物抗病营养教育部重点实验室,四川雅安625014

出　　处：《中国畜牧杂志》2011年第1期41-44,共4页Chinese Journal of Animal Science

基　　金：教育部创新团队研究项目(IRT0555-5)

摘　　要：试验选用24头大约克母猪,随机分为2个处理组,每组12个重复,研究妊娠期添加叶酸对新生仔猪肝脏抗氧化能力及其与叶酸代谢相关基因表达量的影响。对照组饲喂基础日粮(叶酸1.3 mg/kg),基础日粮为典型的玉米-豆粕型日粮,在基础日粮中添加叶酸构成处理组日粮(叶酸30 mg/kg),试验从母猪配种开始直至分娩。结果表明:妊娠期间增加母猪叶酸摄入量显著提高仔猪血清叶酸含量(P<0.01),极显著降低血清同型半胱氨酸含量(P<0.01)。叶酸添加组仔猪肝脏中总抗氧化能力(P<0.05)、谷胱甘肽过氧化物酶(P<0.05)和总超氧化物岐化酶活力(P<0.05)显著高于对照组。仔猪肝脏丙二醛和一氧化氮含量在添加叶酸之后显著下降(P<0.05)。添加叶酸显著提高仔猪肝脏中丝氨酸羟甲基转移酶基因表达量(P<0.05),下调T-蛋白基因mRNA水平(P<0.05)。由此可见,通过母体添加叶酸可显著改善仔猪肝脏抗氧化功能,并影响叶酸代谢相关基因表达量。Twenty-four gilts（Yorkshire） were randomly allotted to 2 groups with 12 replications to study the effects of maternal folic acid supplementation on hepatic antioxidant capacity and expression patterns of genes involved in folic acid metabolism in newborn piglets during gestation period.The control group was fed with basal diet（folic acid 1.3 mg/kg）,which was the typical corn-soybean meal.Folic acid was added into the basal diet as the experimental group diet（folic acid 30 mg/kg）.The trial duration was last from mating to delivery.The results showed that maternal folic acid supplementation increased serum folic acid content of piglets（P〈0.01） and reduced serum homocysteine content（P〈0.01） significantly during the gestational period.Compared with the control group,the activities of hepatic T-AOC（P〈0.05）,GSH-Px（P〈0.05） and SOD（P〈0.05） in piglets of experimental group were increased,but the concentrations of MDA and NO were reduced significantly（P〈0.05） after folic acid addition.Maternal folic acid supplementation increased the expression levels of cSHMT（P〈0.05） and decreased the mRNA levels of T-protein（P〈0.05）.The results revealed that maternal folic acid supplementation could improve hepatic antioxidant capacity in piglets and altered expression patterns of genes involved in folic acid metabolism.

关 键 词：叶酸 抗氧化 基因表达 母猪 

分 类 号：S828.5[农业科学—畜牧学]