柱前衍生化GC法测定旱芹非淀粉多糖中各单糖组分含量  

Determination of the contents of monosaccharide components in non-starch polysaccharides of aerial part of Apium graveolens L. by pre-column derivatization GC

作　　者：谭亮[1] 杨洋[1] 张琦[2] 霍鑫[1] 毕开顺[1] 贾英[2] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2011年第1期40-46,共7页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立测定旱芹非淀粉多糖中各单糖组分含量的柱前衍生化GC法。方法非淀粉多糖经衍生化生成糖腈乙酯,采用GC法进行分析。色谱柱:DB-17柱(30 m×0.25 mm,0.25μm);柱温:程序升温;载气:N2;进样温度:270℃;进样方式:不分流;检测器温度:270℃;进样量:1.0μL。结果水不溶性非淀粉多糖(insoluble non-starch polysaccharides,INSP)水解后得到7种单糖,分别是:L-(+)-鼠李糖、L-(+)-阿拉伯糖、D-(+)-岩藻糖、D-(+)-木糖、D-(+)-甘露糖、D-(+)-葡萄糖和D-(+)-半乳糖;水溶性非淀粉多糖(soluble non-starch polysaccharides,SNSP)水解后得到6种单糖,除无D-(+)-岩藻糖外,其余均同INSP水解后单糖。结论本方法准确度高,重现性好,可用于测定旱芹水溶性和水不溶性非淀粉多糖中各单糖组分的含量。Objective To establish pre-column derivatization GC method for determination of monosaccharide component of non-starch polysaccharides of the aerial part of Apium graveolens L.Methods The derivatives were determined by GC and DB-17 column（30 m×0.25 mm,0.25 μm）was used.The inlet temperature was maintained for 270 ℃.The column oven was begun at 180 ℃,then programmed from 180 ℃ to 225 ℃ at 3 ℃·min^-1,hold for 2 min,then programmed from 225 ℃ to 260 ℃ at 6 ℃·min^-1,and finally,hold for 7 min.Manner of sample injection was splitless.Detector temperature was maintained at 270 ℃.Sample amount was 1.0 μL.Results Seven monosaccharides L-（＋）-rhamnose,L-（＋）-arabinose,D-（＋）-fucose,D-（＋）-xylose,D-（＋）-mannose,D-（＋）-glucose and D-（＋）-galactose were determined in INSP.Except D-（＋）-fucose,other six monosaccharides were the same as from INSP in SNSP.Conclusions The method is accurate and reproducible,which is suitable for the determination of monosaccharide component in soluble and insoluble non-starch polysaccharides in Apium graveolens L.

关 键 词：柱前衍生化 气相色谱法 旱芹 非淀粉多糖 单糖组分 含量测定 

分 类 号：R917[医药卫生—药物分析学]