心力衰竭大鼠肾脏α_1及β肾上腺素受体各亚型表达的改变  

Changes of expression of renalα_1-adrenergic receptor and β-adrenergic receptor subtypes in rats with heart failure

作　　者：刘飞[1] 李艳芳[1] 曹芳芳[1] 张玲姬[1] 白雪源[2] 吕杨[2] 

机构地区：[1]首都医科大学附属北京安贞医院心内科,100029 [2]解放军总医院肾内科

出　　处：《中华老年心脑血管病杂志》2011年第1期24-26,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：中国中西医结合学会科研基金(2008-01)

摘　　要：目的探讨大鼠心力衰竭时肾脏α_1肾上腺素受体(α_1-AR)和β肾上腺素受体(β-AR)各亚型表达水平的变化。方法选择雄性Wistar大鼠35只,随机分为对照组10只、假手术组10只,其余大鼠制作心肌梗死后心力衰竭模型,成功9只为模型组。取大鼠肾脏皮质,采用Western blot法分别测定各组大鼠α_1-AR亚型(α_(1)-AR、α_(1B)-AR、α_(1D)-AR)和β-AR亚型(β_1-AR、β_2-AR)表达水平。结果与假手术组比较,模型组大鼠α_(1A)-AR、β_1-AR表达明显下调,差异有统计学意义(P<0.01);各组大鼠α_(1B)-AR、α_(1D)-AR、β_2-AR比较,差异无统计学意义(P>0.05)。结论心力衰竭时肾脏α_(1A)-AR和β_1-AR明显下调,可能与交感神经系统激活有关;而β_2-AR表达水平无明显变化,可能为代偿β_1-AR下调带来的不利影响,以维持肾脏有效灌注,保护肾脏功能。Objective To explore the changes of expression of renal α1- adrenergic receptor（α1-AR） and β- adrenergic receptor（β-AR） subtypes in rats with heart failure. Methods Thirty-five male Wistar rats were randomly divided into three groups： control group （n = 10）, sham operation group （n = 10）,heart failure model group （n = 9 ,heart failure model was established successfully in 9 of 15 male Wistar rats）. The protein levels of al-AR and β1-AR subtypes of renal cortex were measured by Western blot in each group. Results Compared with sham operation group,expression of alA-AR and β1-AR were decreased significantly in heart failure model group （P 〈0.01）. The expression levels of α1B-AR, α1D-AR and β2-AR showed no difference between different groups （P〈0.05）. Conclusion The downregulation of renal α1A-AR and 13,-AR may be associat- ed with the activation of sympathetic nervous system. No significant change in expression level of β2-AR may compensate for the downregulation of βl-AR,in order to maintain effective perfusion and protect normal physiological function of kidney.

关 键 词：心力衰竭 受体 肾上腺素能Α1 受体 肾上腺素能Β 交感神经系统 肾 印迹法 蛋白质 

分 类 号：R541.6[医药卫生—心血管疾病]