利用热稳定蛋白特异条带鉴别籼粳稻的方法研究  

Method for Identifying Indica-Japonica Rice by Using Specific Heat-Stable Protein Bands

作　　者：付深造[1] 辛霞[1] 张志娥[1] 陈晓玲[1] 辛萍萍[1] 卢新雄[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081

出　　处：《植物遗传资源学报》2011年第1期19-24,共6页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划项目(2006BAD13B10);农业部财政专项(2130135)

摘　　要：以85份栽培稻为材料,利用SDS-聚丙烯酰胺凝胶电泳和蛋白免疫印迹技术分析籼稻和粳稻热稳定蛋白的表达差异,探讨利用特异蛋白来建立籼粳稻的鉴别方法。结果表明,在籼粳稻品种之间存在热稳定蛋白的差异表达,尤其在分子量约42～47kD范围。其中45.2kD条带(BandⅠ)和46.5kD(BandⅡ)条带为典型粳稻特异蛋白(Os03g0168100)的标志带,42.0kD条带(BandⅢ)为典型籼稻特异蛋白(OsI_10172)的标志带。以这3条带作为鉴别方法,并与程氏指数法进行比较,典型籼稻和粳稻的一致度分别为80.0%和86.4%,表明热稳定蛋白标志带法在一定程度上可用于鉴别典型的籼稻和粳稻。Differential expression of heat-stable proteins in eighty-five cultivated rice（Oryza sativa L.）was analyzed by using SDS-PAGE and western blotting,to explore the feasibility of using specific proteins to identify Indica-Japonica rice.The results showed that the heat-stable proteins expressed differentially between Indica and Japonica rice,especially in the molecular weight of about 42-47kD range.Among them,45.2kD band（Band Ⅰ）and 46.5kD band（Band Ⅱ）were marker bands for typical Japonica rice.Both bands matched to Os03g0168100 in NCBI protein databank,42.0kD band（Band Ⅲ,matched to OsI_10172）was marker band for typical Indica rice.Eighty-five Indica and Japonica rice varieties were identified based on the expression pattern of these three bands,and then compared the results with Cheng＇s index.The concordances were 80.0% and 86.4% in typical Indica rice and Japonica rice,respectively.Therefore,the method of hest-stable protein marker bands can be used for identifying typical Indica rice and Japonica rice.

关 键 词：籼稻 粳稻 热稳定蛋白 SDS-聚丙烯酰胺凝胶电泳 蛋白免疫印迹 

分 类 号：S511.2[农业科学—作物学]