瑞芬太尼预处理对大鼠脑缺血再灌注损伤细胞凋亡的影响  

Effects of remifentanil on cell apoptosis in ischemia-reperfusion injury in rat brain

作　　者：王伟[1] 景桂霞[1] 赵新京[1] 王静[2] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,陕西西安710061 [2]西安交通大学医学院第一附属医院神经外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2011年第1期128-130,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨瑞芬太尼预处理对大鼠局灶性脑缺血再灌注损伤中细胞凋亡的影响。方法 SD大鼠20只,体重280～320 g,随机分为2组。瑞芬太尼预处理(R)组(n=10)经股静脉注入瑞芬太尼,速度为0.6μg/(kg.min),每次输注5 min,连续3次,中间间隔5 min;盐水对照(C)组(n=10)经股静脉注入生理盐水,每次输注5 min,连续3次,中间间隔5 min;30 min后对所有动物的右侧颈内动脉用尼龙线线栓法致大脑中动脉阻闭120 min,然后拔出尼龙线恢复再灌注。再灌注2 h后处死动物,取大脑组织标本,原位细胞凋亡法测定细胞凋亡的情况,HE染色观察形态学变化。结果光镜观察发现R组细胞肿胀坏死明显减轻,凋亡细胞及坏死细胞较C组明显减少。再灌2 h后R组凋亡细胞阳性率、TUNEL阳性率较C组减少(P<0.05)。结论瑞芬太尼能减轻大鼠局灶性脑缺血再灌注损伤,抑制神经细胞凋亡坏死,具有脑保护作用。Objective To investigate the effects of remifentanil against cell apoptosis due to ischemia-reperfusion（I/R） injury in rat brain.Methods Twenty healthy male SD rats were randomly assigned to remifentanil（n=10） and control（n=10） groups.The control group rats received saline infusion intravenously with the regime（3×5min infusion with 5min interval）.The rats in remifentanil preconditioning group received remifentanil infusion intravenously at the rate of 0.6μg/（kg·min） with the same regime with those in control group.At 30min after pretreatments,all rats were subjected to the right middle cerebral artery occlusion（MCAO） for 120min and 2h reperfusion.After operation,rats were killed and brains were removed for the detection of apoptotic neurons using TUNEL staining combined with electron microscopic examination.Results The number of apoptotic neurons of remifentanil group was significantly less than that of control group（P0.05）,while inflammatory reaction of remifentanil group was milder than that of control group.Conclusion Remifentanil retreatment decreases neuronal apoptosis induced by I/R,which relieves the brain injury caused by I/R.

关 键 词：瑞芬太尼 脑缺血 再灌注损伤 细胞凋亡 

分 类 号：R971.2[医药卫生—药品]