RP-HPLC同时测定温胆汤中甘草苷、柚皮苷、橙皮苷和甘草酸  

Determination of liquiritin,naringin,hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC

作　　者：许栋明[1] 程可建 

机构地区：[1]科技部科技型中小企业技术创新基金管理中心,北京100038 [2]北大世佳研究中心,北京100084

出　　处：《中国中药杂志》2011年第1期45-47,共3页China Journal of Chinese Materia Medica

摘　　要：目的：建立HPLC同时测定温胆汤中甘草苷、柚皮苷、橙皮苷和甘草酸含量的方法。方法：DIKMA Diamonsil（2）-C18柱（4.6 mm×250 mm,5μm）;流动相乙腈（A）-0.1%磷酸溶液（B）,线性梯度洗脱;检测波长237,283 nm;柱温25℃;流速1.0 mL.min-1;进样量10μL。结果：甘草苷、柚皮苷、橙皮苷和甘草酸铵的进样量与峰面积,分别在0.019 90.119（r=0.999 7）,0.1801.08（r=0.999 7）,0.1460.873（r=0.999 8）,0.03930.236μg（r=0.999 7）呈良好的线性关系;平均加样回收率依次为97.7%,97.7%,97.1%,98.5%,RSD 1.4%,2.0%,2.0%,1.9%。结论：该方法快速,简便,重复性好,适合于同时测定温胆汤样品中甘草苷、柚皮苷、橙皮苷和甘草酸的含量。Objective： To develop a RP-HPLC method for simultaneous determination of liquiritin,naringin,hesperidin and glycyrrhizic acid in extraction of Wendan formula.Method： DIKMA Diamonsil（2）-C18 column（4.6 mm×250 mm,5 μm） was used at 25 ℃ with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner.The flow rate was set at 1.0 mL·min-1.The detection wavelength was 237,283 nm.Result： The linear responses ranged from 0.019 9-0.119 1 μg for liquiritin（r=0.999 7,n=6）,0.180 0-1.080 0 μg for naringin（r=0.999 7,n=5）,0.145 5-0.873 0 μg for hesperidin（r=0.999 8,n=6）,0.039 3-0.235 5 μg for monoammonium glycyrrhizinate（r=0.999 7,n=6）,respectively.The average recoveries were 97.7% with RSD 1.5% for liquiritin,97.7% with RSD 2.0% for naringin,97.1% with RSD 2.0% for hesperidin and 98.5% with RSD 1.9% for glycyrrhizic acid,respectively.Conclusion： The method is quick,simple and repeatable for simultaneous determination of liquiritin,naringin,hesperidin and glycyrrhizic acid in extraction of Wendan formula.

关 键 词：温胆汤 甘草苷 柚皮苷 橙皮苷 甘草酸 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]