参莲方对大鼠动脉粥样硬化病变形成过程影响的动态观察  

Influence of Shenlian decoction on early lesion of atherosclerosis in rats

作　　者：陈丽娜 李玉洁[2] 杨庆[2] 翁小刚[2] 周立运[2] 朱晓新[2] 

机构地区：[1]国家中医药管理局科技司,北京100027 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2011年第1期66-71,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(30973901);国家直属科研院所技术开发研究专项资金(NCSTE-2007-JKZX-301);重大新药创制综合性中药新药研究开发技术大平台项目(2009ZX09301-005-2-4);中国中医科学院自主选题项目(ZZ20090207)

摘　　要：目的:建立大鼠动脉粥样硬化(AS)模型,观察动脉粥样硬化早期病变的动态变化过程和参莲方的干预作用。方法:240只大鼠随机分为空白对照组、模型组、阳性药组(阿司匹林0.027 g.kg-1)、参莲方高、中、低剂量组(0.675,1.35,2.70 g.kg-1)。采用球囊损伤法+半量高脂饲料建立AS模型,并给予参莲方治疗。HE法观察血管病理学变化,采用酶比色法、清除法、硝酸还原酶法测定TC,TG,LDL-C,HDL-C,NO水平的变化,放免法观察ET,TXB2,6-keto-PGF1α,TNF-α,IL-6及IL-8水平的变化。结果:参莲方各剂量组和同阶段模型组相比血管损伤均有明显改善,内膜炎细胞浸润、中膜增厚和平滑肌增殖减轻,第8周末参莲方组无明显脂质条纹出现。参莲方各剂量组血清TG,NO,TXB2的含量明显降低(P<0.05);高剂量组可明显降低血清TNF-α,IL-6,IL-8水平(P<0.05)。结论:参莲方能够通过调节血脂,改善血管内皮功能,减少血液循环中炎症介质的释放,抑制AS病变的形成和发展。Objective： To establish the atherosclerosis models（AS） in rats and observe the change process of AS and Shenlian decoction’s pharmacological effect.Method： Two hundreds and forty rats were randomly divided into blank control group,model group,positive medicine group,the high-dose,middle dose and low-dose Shenlian decoction groups.Vascular pathological changes were observed by the methods of HE stain.The levels of TC,TG,LDL-C,HDL-C,NO in serum were determined respectively by enzyme colorimetric,clear and nitrate reductase methods.ET,TXB2,6-keto-PGF1α,TNF-α,IL-6 and IL-8 levels in serum were detected by radioimminoassay.Result： Compared with the model group,vascular lesion of different doses of Shenlian decoction groups were significantly improved,the TG,NO,TXB2 levels in serum were significantly lower（P〈0.05）,while high-dose Shenlian decoction can significantly reduce TNF-α,IL-6,IL-8 levels in serum（P〈0.05）.Conclusion： Shenlian decoction can inhibit the formation and development of AS by regulating blood lipids,improving endothelial function and reducing inflammatory factors release.

关 键 词：参莲方 动脉粥样硬化 炎症 

分 类 号：R285.5[医药卫生—中药学]