小麦光周期基因Ppd-D1的单核苷酸多态性  

Ecotilling Detection of SNP in Photoperiod Related Gene Ppd-D1 of Wheat

作　　者：陈亮[1] 张娜[1] 胡银岗[1,2,3] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]陕西省农业分子生物学重点实验室,陕西杨凌712100 [3]国家小麦改良中心杨凌分中心,陕西杨凌712100

出　　处：《麦类作物学报》2011年第1期15-20,共6页Journal of Triticeae Crops

基　　金：国家"863"计划重点项目子课题(2006AA100201;2006AA100223);科技部"973"计划前期项目(2006CB708208);澳大利亚ACIAR项目(CIM/2005/111)

摘　　要：为了解小麦光周期基因Ppd-D1编码区单核苷酸多态性(Single Nucleotide Polymorphism,SNP),采用琼脂糖凝胶电泳Ecotilling技术,对108份小麦品种中光周期基因Ppd-D1的核心编码区进行了SNP检测。结果表明,红芒麦、宁春27和红芒麦2具有相同的酶切带型,其目的序列与对照相比存在两处单碱基突变(C/T;G/A);坝农1号、定西35、QW6285等7份材料具有相同的酶切带型,其序列与对照相比含有一个5bp的缺失。初步分析表明,这三处SNP与小麦光周期敏感性没有直接的对应关系,但它们对小麦光周期敏感性产生了强度上的影响,加强了材料对光照长度的敏感性,一定程度上延迟了抽穗。本研究结果也说明琼脂糖凝胶电泳Ecotilling技术在小麦Ppd-D1基因SNP检测中具有良好的可行性。In order to find the SNP（Single Nucleotide Polymorphism） in the coding region of photoperiod gene Ppd-D1 of wheat,and to optimize the Ecotilling systerm based on agarose gel detection,the SNPs in the key coding region of photoperiod gene Ppd-D1 of 108 wheat varieties were screened with the established agarose-gel Ecotilling technique.The results showed that the same digestion patterns were observed in varieties of Hongmangmai,Hongmangmai2 and Ningchun27,and two SNPs（C/T;G/A） in the target region were then confirmed by compare with the control.The same digestion patterns were alos observed in varieties of Banong1,Dingxi35,QW6285 and other four materials,sequencing analysis confirmed there was a 5bp deletion in their target region while compared with the control.Further analysis showed there was no direct correlation between the SNPs in Ppd-D1 revealed here with the photoperiod response of wheat,but some variation on the heading date and enhanced the sensitive of daylight were observed.

关 键 词：小麦 光周期基因Ppd-D1 单核苷酸多态性 

分 类 号：S512.1[农业科学—作物学] S330