HMW-GS分子标记多重PCR体系的建立及新疆春小麦的检测  

Establishment of Multiplex PCR based on Molecular Markers for HMW-GS and Identification of Xinjiang Spring Wheat

作　　者：王晓龙[1] 王亮[2,3] 尉倩[1] 穆培源[2,3] 范锋贵[1] 徐红军[2,3] 梁强[1] 任万杰[1] 张晓科[1,3] 

机构地区：[1]西北农林科技大学农学院/国家小麦改良中心杨凌分中心,陕西杨凌712100 [2]新疆农垦科学院作物研究所,新疆石河子832000 [3]谷物品质与遗传改良兵团重点实验室,新疆石河子832000

出　　处：《麦类作物学报》2011年第1期41-46,共6页Journal of Triticeae Crops

基　　金：新疆兵团科技支疆计划项目(2008ZJ10);兵团农业科技攻关计划项目(2009GG05);农业部"948"项目(2006-G2);国家小麦产业技术体系建设专项(nycytx-03);西北农林科技大学唐仲英育种基金项目

摘　　要：为给新疆优质春小麦品种选育提供参考依据,利用小麦优质高分子量谷蛋白亚基(HMW-GS)基因的特异性标记,基于17份已知HMW-GS组成的品种(系),构建了3套多重PCR,体系Ⅰ可用于同时检测AxNull和Dx5基因,体系Ⅱ可同时检测Ax2*和By8基因,体系Ⅲ可同时检测Bx14和Dx5基因;用3套多重PCR体系分别检测17份小麦品种,其结果与SDS-PAGE检测结果完全一致,表明建立的3套多重PCR体系稳定可靠,可用于小麦品种优质HMW-GS基因聚合育种。利用此体系对85份新疆春小麦品种进行分析表明,AxNull和Ax1的频率均为24.7%,Ax2*为50.6%,By8为48.2%,Bx14(+By15)为0%,Dx5(+Dy10)为34.1%。High-molecular-weight glutenin subunits（HMW-GS） are highly correlated with the processing quality of common wheat,therefore,the establishment and use of their multiplex PCR systems are of great importance in selection of quality characteristic of wheat.According to the genes coding for good quality HMW-GS,three types of multiplex PCR were established and validated on 17 Chinese wheat cultivars and advanced lines with known HMW-GS composition.The first multiplex PCR was used to simultaneously detect genes AxNull and Dx5,the second one was to detect the genes Ax2＊ and By8,and the third one was to detect Bx14 and Dx5.Those multiplex PCR were used to detect HMW-GS genes from Xinjiang spring wheat cultivars.The frequencies of genes AxNull,Ax1,Ax2＊,By8 and Dx5（＋Dy10）in 85 Xinjiang spring wheat cultivars were 24.7%,24.7%,50.6%,48.2% and 34.1%,respectively.In addition,the gene Bx14（＋ By15）was not detected in this study.The results are very important in understanding the genetic basis of HMW composition and promotion of high quality wheat cultivars in Xinjiang region.

关 键 词：小麦 高分子量谷蛋白亚基 多重PCR 

分 类 号：S512.1[农业科学—作物学] S331