河南省小麦条锈菌特异性SSR标记的筛选  

Screening of the SSR Markers Specific to Puccinia striiformis f.sp. tritici from Henan Province

作　　者：代君丽[1] 刘珂[1] 于思勤[2] 李洪连[1] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]河南省植保植检站,河南郑州450002

出　　处：《麦类作物学报》2011年第1期166-169,共4页Journal of Triticeae Crops

基　　金：国家"十一五"科技支撑计划重大项目粮食丰产科技工程(2006BAD02A07-1)

摘　　要：为了简便地进行小麦条锈菌的早期检测,在前期工作的基础上,以小麦条锈病、叶锈病、白粉病的病叶和健康叶片为实验材料,采用来自条锈菌的SSR引物进行河南省小麦条锈菌的分子检测。结果表明,在所用的条锈菌特异性SSR引物中,发现有4对引物能够将条锈菌和其他的病原菌区分开来。通过对来自河南省不同地区的小麦条锈病和叶锈病病叶的鉴定,其中2对引物RJ3、RJ21的稳定性和可重复性均较好,表明这2对引物可以用于小麦条锈菌的分子检测。Based on the previous research,diseased leaves with wheat stripe rust,wheat leaf rust,wheat powdery mildew and normal leaves were used as experimental materials to detect Puccinia striiformis f.sp.tritici in Henan province using SSR technique.The results showed that 4 pairs of SSR primers could identify Puccinia striiformis f.sp.tritici from others.The detection with primers RJ3 and RJ21 to the materials from different regions of Henan province was stable and repeatable,and could be used to distinguish Puccinia striiformis f.sp.tritici from Puccinia recondite f.sp.tritici.

关 键 词：小麦 条锈菌 SSR 

分 类 号：S512.1[农业科学—作物学] S332.2