实时荧光定量-PCR和酶联免疫吸附法检测巨细胞病毒-IgM抗体的比较  

Comparison of cytomegalovirus-IgM detection by real time-PCR and enzyme linked immunoabsorption assay

作　　者：范文勇[1] 

机构地区：[1]广东省深圳市宝安区人民医院,广东深圳518101

出　　处：《中国医药导报》2011年第2期83-84,共2页China Medical Herald

摘　　要：目的:比较实时荧光定量-PCR和酶联免疫吸附法检测巨细胞病毒-IgM抗体水平的敏感性及准确率。方法:收集108例孕前筛查妇女,同时采用实时荧光定量-PCR和酶联免疫吸附法检测外周血巨细胞病毒-IgM抗体水平,并对这两种检测方法的阳性率进行比较。结果:实时荧光定量-PCR法阳性率为74%,酶联免疫吸附法为57%,两者差异有统计学意义(P<0.05);18例荧光定量-PCR法阳性的妇女血清巨细胞病毒-IgM拷贝数低于10-4/ml。结论:实时荧光定量-PCR与酶联免疫吸附法相比,对巨细胞病毒-IgM抗体检测具有较高的敏感性和准确率,值得临床推广使用。Objective： To compare the sensitivity and accuracy of cytomegalovirus-IgM（CMV-IgM） detection by real-time PCR and enzyme linked immunoabsorption assay（ELISA）.Methods： We enrolled 108 females who were underwent pre-pregnancy evaluation and evaluated peripheral blood CMV-IgM level by both real time-PCR and ELISA.Positive rates of these two methods were compared.Results： Positive rate in real time-PCR was 74% while in ELISA was 57%,and there was a significant difference between these two methods（P0.05）.Copy number in 18 positive cases with real-time PCR detection was less than 10-4/ml.Conclusion： The sensitivity and accuracy of CMV-IgM detection with real-time PCR is higher than ELISA,and real-time PCR deserves broadly applied in clinic.

关 键 词：实时荧光定量-PCR 酶联免疫吸附法 巨细胞病毒-IgM抗体 

分 类 号：R446.5[医药卫生—诊断学]