甲氧滴滴涕对雌性大鼠性腺毒性作用及其机制研究  

Research of relation between female gonad toxicity of methoxychlor on rats and the expression of estrogen receptor in uterus and ovary

作　　者：常飞[1] 汪惠文[2] 陈必良[3] 马向东[3] 

机构地区：[1]兰州军区总医院妇产科,730050 [2]兰州军区总医院麻醉科,730050 [3]第四军医大学附属西京医院妇产科,西安710033

出　　处：《重庆医学》2011年第1期9-11,共3页Chongqing medicine

摘　　要：目的探讨甲氧滴滴涕(MXC)对雌性大鼠性腺毒性的作用机制。方法成年雌性清洁级SD大鼠40只,按体质量随机分成4组,染毒剂量分别为0、16、326、4 mg/kg。每天以1 mL/100 g体质量腹腔注射染毒,连续20 d。结果高、中剂量染毒组大鼠动情周期延长,与对照组比较差异有统计学意义(P<0.05)。各染毒组大鼠子宫脏器系数、卵巢脏器系数以及子宫、卵巢间质雌激素受体(ER)的表达阳性率与对照组比较差异均无统计学意义(P>0.05)。结论在本实验剂量条件下,MXC可能不是通过影响ER表达而产生性腺毒性。Objective To study the relation between female gonad toxicity of methoxychlor on rats and the expression of estrogen receptor（ER） in uterus and ovary.Metheds 40 SD female rats were randomly divided into 4 groups base on body weight and were injected subcutaneously by the 4 dose levels（o,16,32,64 mg/kg body weight） for 20 days.Vaginal smears of rats were taken to determine estrous cycle.The rats were sacrificed on the day of estrus stage.Body weights,estrus stage,organ weights and organ coefficients of ovary were observed.Serum follicle stimulating hormone（FSH）,leteinizing hormone（LH）,estradiol（E2） and progestereone（P） were detected with the radio immunoassays.Results The period of estrus cycle of intermediate and high concentration groups were longer than normal group（P〈0.05）.The organ coefficients of uterus,ovary and the positive arte of ER of experiment groups was similar to normal group（P〉0.05）.Conclusion MXC in experimental dose may induce the disorder of hypothalamic-pituitary-ovarian axis and decrease the expression level of FSH,however,this dose can not change the expression level of ER secreted from ovarian and uterus,and increase the toxicities of female gonad.

关 键 词：甲氧氯 受体 雌激素 性腺毒性 

分 类 号：R114[医药卫生—卫生毒理学]