-80℃冰冻保存对血小板CD_(62)p表达的影响  

Expression of CD_(62)P of platelet cryo-preservation at -80℃

作　　者：何丽娟[1] 邓为彬[2] 李永萍[1] 

机构地区：[1]大理学院附属医院血液肿瘤科,云南大理671000 [2]浙江省嘉兴市第二医院,浙江嘉兴314000

出　　处：《海南医学》2011年第2期10-11,共2页Hainan Medical Journal

基　　金：大理学院科研项目(编号:2007X11)

摘　　要：目的初步探讨-80℃冰冻保存血小板1个月对血小板CD62p表达的影响。方法采用流式细胞仪检测12例健康献血者-80℃冰冻1个月前后血小板CD62p的表达率。结果血小板CD62p表达率冰冻前后对比差异均无统计学意义(P>0.05)。结论血小板加终浓度为5%DMSO,在-80℃条件下冰冻保存1个月,对血小板的活化功能无明显影响。Objective To investigate and analyze the CD62p expression of platelet at-80℃ froze preserving.Methods Using flow cytometry（FCM） detection,12 healthy donors CD62p express rate of platelet before-80℃ freezing and one month after freezing.Results CD62p expression rate freeze in platelet showed no statistically significant difference before and after freezing.Conclusion Add the final concentration of 5% DMSO,at-80℃ under the conditions of frozen preservation of one month,platelet activation function has no obvious effect.

关 键 词：血小板 冰冻保存 二甲基亚砜 CD62P表达 

分 类 号：R558[医药卫生—血液循环系统疾病]