甘草酸二铵对博莱霉素致肺纤维化大鼠的治疗作用  

The therapeutical effect of diammcnium glycyrrhizinate in rat model of pulmonary fibrosis by bleomycin

作　　者：殷宗宝[1] 邓超[1] 李英[1] 李海军[1] 陈栩栩[1] 程启慧[1] 

机构地区：[1]海南省海口市人民医院重症医学科,570208

出　　处：《重庆医学》2011年第2期122-124,F0003,共4页Chongqing medicine

摘　　要：目的探讨甘草酸二铵治疗博莱霉素致肺纤维化大鼠的作用及其机制。方法将清洁级大鼠40只随机分成对照组、博莱霉素所致大鼠肺纤维化组(模型组)、甘草酸二铵组和地塞米松(DXM)组。DXM组和甘草酸二铵组滴注博莱霉素致肺纤维化动物模型第28天开始,连续14 d腹腔内分别注射DXM注射液3 mg.kg-1.d-1和甘草酸二铵150 mg.kg-1.d-1;第15天取左肺下叶用于免疫组化检测转化生长因子β1(TGF-β1)、γ干扰素(IFN-γ)表达,右肺下叶经HE染色病理观察。用ELISA双抗体夹心法测定各组大鼠血清TGF-β1、白细胞介素4(IL-4)、IFN-γ含量。结果 (1)甘草酸二铵组与DXM组大鼠肺纤维化程度均较轻微;(2)模型组大鼠肺组织TGF-β1染色呈强阳性反应,IFN-γ染色较淡,而甘草酸二铵组与DXM组TGF-β1、IFN-γ染色及定量表达则相反(P<0.01);且甘草酸二铵组与DXM组相比,TGF-β1定量表达有差异(P<0.05)。(3)模型组大鼠血清TGF-β1、IL-4含量较高,IFN-γ含量较低;而甘草酸二铵组和DXM组则相反(P<0.01)。结论甘草酸二铵可以下调大鼠肺组织TGF-β1、IL-4的表达,降低其血清含量;上调肺组织IFN-γ的表达,增加其血清含量,从而减轻肺纤维化。Objective To investigate the therapeutical effect of diammonium glycyrrhizinate in rat model of pulmonary fibrosis by Bleomycin.Methods Rats were randomly divided into 4 groups：pulmonary fibrosis group,NS group,DXM treatment group,glycyrrhizin treatment group.All rats in the treatment groups were intraperitoneal injection after 28 days when rats were produced pulmonary fibrosis,which lasted for 14 days.The treatment dose was DXM 3mg·kg-1·d-1,glycyrrhizin dose 150 mg·kg-1·d-1.After the 15th days,Lung tissues were obtained,and the left lung were measured by immunity class TGF-β1,IFN-γ;the right lung was made into slice which cell apoptosis was detected.The blood was obtained to measure TGF-β1,IFN-γ and IL-4 by ELISA.Results（1） The pulmonary alveolitis and fibrosis of glycyrrhizin group and DXM group were signifieantly lighter;（2） Stronger TGF-β1 protein expression was detected in bronehiolar epitheliums,poorer IFN-γ in BLM group.But the oppositions were in glycyrrhizin group and DXM group（P0.01）.The difference of TGF-β1 was obvious between DXM group and glycyrrhizin group（P0.05）.（3）The TGF-β1 and IL-4 levels of the serum in BLM group were higher than all the treatment groups（P0.01）,but the IFN-γ level in BLM group was poorer than DXM group and glycyrrhizin group（P0.01）.Conclusion The study shows glycyrrhizin can reduce TGF-β1 and IL-4 protein expressions and the TGF-β1 and IL-4 levels in BLM group.But IFN-γ protein expression and the IFN-γ level were stronger in glycyrrhizin group.Those evidenees suggest that glycyrrhizin has strong ability to antifibrosis.

关 键 词：肺纤维化 干扰素Ⅱ 转化生长因子Β1 白细胞介素4 博莱霉素 甘草酸二铵 

分 类 号：R563[医药卫生—呼吸系统]