复方中草药对奥尼罗非鱼血液非特异性免疫功能的影响  

Effects of a Chinese Herbal Compound on Nonspecific Immunity in Blood of Tilapia

作　　者：张照红[1] 林旋[1] 张伟妮[1] 王全溪[1] 李辉选[1] 姚华阳[1] 王寿昆[1] 

机构地区：[1]福建农林大学动物科学学院,福建福州350002

出　　处：《水产科学》2011年第1期1-5,共5页Fisheries Science

基　　金：农业公益性行业科研专项经费项目子项目(20080313);国家大学生创新性实验计划项目(091038913)

摘　　要：在基础饲料中添加3个不同水平的复方中草药分别饲养奥尼罗非鱼,旨在探讨复方中草药对奥尼罗非鱼血液非特异性免疫功能的影响。挑选质量(36±5)g的健康奥尼罗非鱼,共480尾,随机分为8组,每组60尾,每组设置1个平行。分别用含有0‰(对照组)、0.5‰、1.0‰、1.5‰复方中草药的饲料喂养60 d,每15 d分别取血样,用于测定血液白细胞吞噬能力,血液硝基四氮唑蓝阳性细胞数量,血清中超氧化物歧化酶、过氧化氢酶、溶菌酶的活力。结果表明,与对照组相比,1.0‰、1.5‰添加组在第30 d和45 d可以显著或极显著提高奥尼罗非鱼血液白细胞吞噬能力、硝基四氮唑蓝阳性细胞数量及其血清中超氧化物歧化酶、过氧化氢酶、溶菌酶的活力。综合奥尼罗非鱼各项血液非特异性指标表明,在基础日粮中添加1.0‰、1.5‰复方中草药可以显著提高奥尼罗非鱼的非特异性免疫功能。Four hundred and eighty healthy tilapia with body weight of（36±5） g were randomly divided into 8 groups at a rate of 60 individuals per group and fed diets containing 0‰（control group）,0.5‰,1.0‰,and 1.5‰ of a Chinese herbal compound for 60 days with duplication.Meanwhile,tilapia blood were sampled every 15 days to determine phagocytosis activity and the amount of NBT～positive cells in the blood and activities of superoxide dismutase（SOD）,catalase（CAT）,and lysozyme（LSZ）in the serum.The results showed that 1.0‰,and 1.5‰ of Chinese herbal compound led to increase in phagocytosis activity,the amount of NBT～positive cells and the activities of SOD,CAT,and LSZ significantly or very significantly on the 30th day and the 45th day in the experiment compared with the control diet,indicating that the diet containing 1.0‰,and 1.5‰ Chinese herbal compound significantly improved the tilapia non～specific immunity.

关 键 词：复方中草药 奥尼罗非鱼 吞噬能力 硝基四氮唑蓝阳性细胞 血清酶 

分 类 号：S965.125[农业科学—水产养殖]