创伤弧菌、溶藻弧菌外膜蛋白特性的比较研究  

Comparative Characteristics of Outer Membrane Proteins in Bacteria Vibrio vulnificus and V.alginolyticus

作　　者：田丁[1] 林天龙[1] 许斌福[1] 

机构地区：[1]福建农业科学院生物技术研究所,福建福州350003

出　　处：《水产科学》2011年第1期27-30,共4页Fisheries Science

基　　金：国家"863"计划项目(2006AA100208);福建省科技重点项目(2008N0023)

摘　　要：对用Sarkosyl法分离的创伤弧菌、溶藻弧菌、副溶血弧菌、鳗弧菌的外膜蛋白进行了初步的比较分析。这4种弧菌外膜蛋白的SDS-PAGE和Western blotting的图谱有相似性亦有差异。SDS-PAGE显示,4种弧菌中除副溶血弧菌外均能分离到47、38 ku的外膜蛋白;创伤弧菌和溶藻弧菌存在4种共同的外膜蛋白,大致相对分子量为47、40、38、36 ku;溶藻弧菌和鳗弧菌有75、47、43、38 ku的4种共同的外膜蛋白。免疫印迹结果显示,兔抗创伤弧菌外膜蛋白血清能与创伤弧菌外膜蛋白、溶藻弧菌外膜蛋白、鳗弧菌外膜蛋白进行免疫反应,其中与溶藻弧菌外膜蛋白的反应性相对较强,有3条清晰的反应发色带,大致相对分子量为38、30、29 ku;而38 ku的外膜蛋白是创伤弧菌和溶藻弧菌共同的免疫反应带;说明这2种弧菌可能有共同的特异性抗原。The characteristics of outer membrane proteins（OMPs）isolated from cell membrane fraction of Vibrio vulnificus,V.alginolyticus,V.anguillarum,and V.parahaemolyticus by Sarkosyl method were analyzed by SDS-PAGE.The V.vulnificus,V.alginolyticus,and V.anguillarum strains were found to have the common major OMPs such as 47 ku,and 38 ku while V.vulnificus and V.alginolyticus strains had common major protein bands with molecular weights of 47 ku,40 ku,38 ku,and 36 ku.V.alginolyticus and V.anguillarum strains had common 4 membrane proteins containing 75 ku,47 ku,43 ku,and 38 ku.OMPs of strain FJ03-X2 effectively stimulated the rabbit to produce immunity response,and the titer of the antiserum was above 102400 by enzyme-linked immunosorbent assay（ELISA）,indicating that the OMPs had strongly immunogenicity.The Western-blot with rabbit antiserum against FJ03-X2 OMPs revealed that the OMPs of strain FJ03-X2 had molecular weights from 66 ku to 30 ku,and the OMPs of V.alginolyticus molecular weights of 38 ku,34 ku,30 ku,and 29 ku,the OMPs of V.anguillarum molecular weight of 26 ku,the OMPs of V.parahaemolyticus and Aeromonas hydrophila were not detected.The band of 38 ku had visible positive reaction in V.vulnificus and V.alginolyticus.The results revealed that the 38 ku OMP was the common special antigen of the two vibrios.

关 键 词：创伤弧菌 溶藻弧菌 外膜蛋白 

分 类 号：Q936[生物学—微生物学]