复发性外阴阴道念珠菌病患者及性伴侣带菌情况分析  

Analysis of yeast species from patients with recurrent vulvovaginal candidiasis and patients' sexual partners

作　　者：宗利丽[1] 王彩丽[1] 曾俊[1] 王玉凤[1] 赵欣[1] 毛婷[1] 

机构地区：[1]南方医科大学中西医结合医院妇产科,广东广州510315

出　　处：《基础医学与临床》2011年第1期6-8,共3页Basic and Clinical Medicine

基　　金：国家高技术研究发展计划(863计划)(102-09-03-06)

摘　　要：目的研究复发性外阴阴道念珠菌病(RVVC)复发相关因素。方法选择55例RVVC患者及其中45位患者性伴侣,取阴道、肛门、口腔及龟头分泌物分离念珠菌,采用ITS1区单链构型多态性(SSCP)分析和大亚基26SrDNA基因D1/D2区序列分析相结合的方法进行菌种鉴定,应用微卫星CAI位点的SSCP和基因扫描分析相结合的方法进行白色念珠菌的基因型测定。结果 RVVC患者阴道、肛门、口腔及其性伴侣龟头分泌物的念珠菌培养阳性率分别为100.0%(55/55)、61.8%(34/55)、1.8%(1/55)和60.0%(27/45),各部位念珠菌感染均以白色念珠菌为主,且所占比例无明显差异。同一患者阴道与肛门、阴道与性伴侣龟头分泌物的白色念珠菌基因型符合率分别为86.2%和54.5%。结论肠道及性伴侣龟头携带的念珠菌可能是RVVC复发的重要因素之一。Objective To explore the factors related to the recurrence of vulvovaginal candidiasis（RVVC）.Methods Samples were collected from the discharge of vagina,rectum,oral cavity of 55 patients and the discharge of balanus in their 45 sexual partners.Using SSCP analysis of internal transcribed spacer（ITS） region and sequence analysis of the 26S rDNA D1/D2 domain to identify the species of C.albicans of the samples.Genotypes of C.albicans strains were determined with use of SSCP combined to GeneScan analysis of microsatellite CAI of C.albicans.Results The positive rate of vagina,oral and rectal Candida species colonization in the 55 women and 45 men was 100.0%（55/55）,61.8%（34/55）,1.8%（1/55） and 60.0%（27/45）,respectively.And the percentage of Candida strains among the vagina,rectum and balanus were concurrent.In the same person,the coincidence of Candida albicans genotype between the discharge from vagina and rectum were 86.2%.54.5% of the Candida albicans genotype of the discharge from the vagina of RVVC patients were same as the discharge from the balanus of her sexual partner.Conclusion The state of having Candida on the rectum or the balanus of sexual partner is one of the potential risk factors of RVVC.

关 键 词：复发性外阴阴道念珠菌病 基因型 单链构象多态性 基因扫描 

分 类 号：R711.3[医药卫生—妇产科学]