GnRH-A促进雌兔FSH与LH分泌及卵巢发育的作用  

GnRH-A enhances FSH and LH secretion,ovarian development of female rabbits

作　　者：巩转娣[1] 魏锁成[2] 韦敏[2] 

机构地区：[1]西北民族大学医学院附属医院 [2]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《基础医学与临床》2011年第1期13-18,共6页Basic and Clinical Medicine

基　　金：国家民委2007年度重点资助项目(MW2007-ZD-027)

摘　　要：目的探讨GnRH类似物(GnRH-A)对FSH与LH分泌及卵巢发育的影响,及GnRH-A调节生育的机制。方法 24只雌兔均分为实验Ⅰ组(EG-Ⅰ)、Ⅱ组(EG-Ⅱ)、Ⅲ组(EG-Ⅲ)和对照组(CG)(n=6),实验组分别于颈背侧皮下注射0.1、0.1和0.05 g/L GnRH-A抗原1.0 mL,EG-Ⅱ和EG-Ⅲ组于20 d加强注射1次。ELISA法测定血清FSH和LH含量。于70 d无菌切取卵巢,光镜和电镜观察。结果 40 d时EG-Ⅱ和EG-Ⅲ组血清FSH和LH浓度均达到峰值,明显高于EG-Ⅰ和CG组的FSH和LH(P<0.01),且EG-Ⅱ组高于EG-Ⅲ组(P<0.05)。EG-Ⅱ组和EG-Ⅲ组卵巢皮质变厚,卵泡纵径和横径均增大,且与剂量相关。实验组卵巢卵泡数增加,生长加快。细胞核、线粒体和线粒体嵴变大,细胞质中的皮质颗粒和分泌物增多,透明带和微绒毛变大。结论 GnRH-A能促进FSH和LH的合成及卵巢与卵泡的发育,加强注射效果更明显。Objective To investigate the effects of GnRH analogue（GnRH-A） on secretion of FSH and LH,ovary histostructure and development of female rabbits.Methods Twenty-four female rabbits（Oryctolagus cuniculus）were divided randomly into experiment groups 1,2,3（EG-Ⅰ,Ⅱ,Ⅲ）and control group（CG）.Rabbit were subcutaneously injected with 1.0 mL GnRH-A antigen respectively at concentrations of 0.1 g/L,0.1 g/L and 0.05 g/L in EG-Ⅰ,EG-Ⅱ and EG-Ⅲ.The rabbits in EG-Ⅱ and EG-Ⅲ were re-injected on 20 d.CG was blank.Ten mL blood was collected on 0,10,20,30,40,50,60 and 70 d.The concentrations of FSH and LH in serum were assayed with ELISA.The ovary was collected aseptically on 70 d.The tissue sections were observed under the optical and electronic microscopes.Results Serum FSH and LH concentrations in EG-Ⅱ and EG-Ⅲ reached the peak on 40 d（20 d after re-injection）.LH concentration in EG-Ⅱ exceeded other 3 groups（P〈0.05）.Numbers of primary follicle increased.The ovarian cortex thickness（OCT）EG-Ⅱ and EG-Ⅲ thickened.The follicle vertical diameter（FVD） and follicle transverse diameter（FTD） enlarged,which was depedend upon the used dosage.GnRH-A could obviously speed up oocyte development by enlarging nucleus and chondriosome,increasing the quantities of mitochondrial cristae.The cortical granules and secretions in cytoplasm also raised.The zona pellucida andmicrovillus of the oocytes broadened and lengthened.Conclusion Injection of GnRH-A may enhance the synthesis and secretion of FSH and LH in female rabbits,and promote the development of ovary and follicle.

关 键 词：促性腺激素释放激素类似物 卵泡刺激素 黄体生成素 卵巢 组织结构 

分 类 号：Q492[生物学—生理学]