α-内收蛋白G460T和血管紧张素原M235T基因多态性与原发性高血压发病风险相关  

Association of α-adducin G460Tand AGT M235Tgene polymorphisms with the risk of essential hypertension

作　　者：钟发德[1] 张莉娜[1] 徐进[1] 张月苗[1] 费丽娟[1] 陈东妮[1] 

机构地区：[1]宁波大学医学院预防医学研究所,浙江宁波315211

出　　处：《基础医学与临床》2011年第1期62-67,共6页Basic and Clinical Medicine

基　　金：宁波农业与社会发展科研攻关项目(2007C10019)

摘　　要：目的探讨α-内收蛋白（α-adducin）G460T、血管紧张素原（AGT）M235T基因多态性与宁波汉族人群原发性高血压（EH）的关系。方法以社区为基础的病例对照研究,用突变分离聚合酶链反应（MS-PCR）及PCR-RFLP技术分别检测305例EH患者和305例血压正常者的α-adducin G460T、AGTM235T基因多态性,并测定各组人群的生化指标。结果α-adducin G460T多态基因型（24.60%、52.78%和22.62%vs28.53%、48.85%和22.62%）和G、T等位基因频率（50.98%和49.02%vs52.95%和47.05%）无统计学差异;AGTM235T多态基因型（18.69%、52.46%和28.85%vs9.51%、41.64%和48.85%,P〈0.01）和M、T等位基因频率（44.92%和55.08%vs30.33%和69.67%,P〈0.01）有显著差异;同时分析AGT基因M235T基因型与α-adducin基因G460T基因型联合作用时,它们患高血压的风险除与α-adducin GG基因型无协同作用外,与其他基因型都有协同作用（P〈0.05）;携带AGTM235T多态TT基因型的个体发生EH的风险增加2.35倍（95%CI=1.69~3.28,P〈0.01）。结论 AGTM235T基因多态性位点TT基因型可能增加EH发病风险;未发现α-adducin G460T基因多态性位点与EH发病风险有统计相关性。Objective To investigate the association between polymorphism of α-adducin G460T,angiotensinogen（AGT） M235T genes and essential hypertension（EH） of Han nationality.Methods MS-PCR and RFLP-PCR were used to detect the polymorphism of α-adducin G460T,AGT M235T genes in 305 EH patients and 305 age-matched,nationality normotensive controls（NT）,biochemical index were also detected.Results The G460T polymorphism of α-adducin gene was not significantly associated with EH,the genotype frequencies were 24.60%,52.78% and 22.62% in the patient group and 28.53%,48.85% and 22.62% in the control group respectively.G and T allele frequencies were 50.98%,49.02% in the patient group and 52.95%,47.05% in the control group;The M235T polymorphism of AGT gene was significantly associated with EH,the genotype frequencies were 18.69%,52.46% and 8.85% in the patient group and 9.51%,41.64% and 48.85% in the control group（P〈0.01）,respectively.M and T allele frequencies were 44.92%,55.08% in the patient group and 30.33%,69.67% in the control group（P〈0.01）;There were statistically significant distribution of M235T genotype of AGT and G46OT genotype of α-adducin combined genes with EH except GG genotype of α-adducin;Individuals carrying TT genotype of AGT M235T gene showed high risk of EH（OR=2.35,95%CI=1.69~3.28,P〈0.01）.Conclusion The TT genotype of AGT M235T gene may be related with high risk of EH,and G460T polymorphism of α-adducin gene has no relation to incidence of EH in Han nationality.

关 键 词：Α-内收蛋白 血管紧张素原 原发性高血压 基因多态性 

分 类 号：R544.1[医药卫生—心血管疾病]