RAPD分子标记对高粱DNA经花粉管导入玉米自交系的验证  

Sorghum DNA introduced into Maizes via pollen tube pathway and molecular verification among their offspring transformed by using RAPD

作　　者：王世伟[1] 杨晓杰[1] 王中革 杨小花[1] 李旭业[3] 冯柠[4] 

机构地区：[1]齐齐哈尔大学生命科学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔第五十七中学,黑龙江齐齐哈尔161005 [3]黑龙江省畜牧研究所,黑龙江齐齐哈尔161005 [4]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2011年第1期54-60,共7页Journal of Qiqihar University(Natural Science Edition)

基　　金：黑龙江省畜牧研究所项目(利用花粉管通道技术改良青贮玉米自交系品质及新品种选育;2008-001)

摘　　要：采用SDS方法纯化高粱总DNA,通过花粉管通道将高粱DNA转化P138、丹改340、7922、06NY-2、组3等5个品种的玉米自交系,后代的玉米种子经随机筛选后在实验室盆栽得到幼苗。使用不同序列的59条RAPD引物,对高粱基因组DNA和导入前后的玉米基因组总DNA进行PCR扩增。结果表明,其中9条引物在后代玉米和玉米原种之间具有RAPD差异,并证明了高粱DNA导入了4个玉米自交系,为今后的玉米自交系花粉管导入法育种的分子检验奠定了基础。The total DNA extracted and purified from the sorghum by new SDS method was introduced into 5 Maize varieties including P138, DG340, 7922,06NY-2, Z3 via a new improved pollen tube pathway. The seeds of different Maizes transformed were chosen by random sampling and their offspring were acquired in potted plant in lab then these offspring DNA was extracted in the same way. The different Maize genome DNA samples as well as the Sorghum genome DNA samples were respectively amplified by using 59 primers of RAPD. The results show that there are different genetic polymorphisms of RAPD in the used 9 primers between different Maize varieties and 4 different transformed Maize varieties. The results also indicate that the Sorghum genome DNA have been introduced into different Maizes. So this paper will lay the rudimentary foundation for success of genetic transformation in Maize breeding and the molecular verification among offspring performed by RAPD in the future

关 键 词：SDS 花粉管通道法 高粱 玉米 RAPD 

分 类 号：S188[农业科学—农业基础科学]