自建抗CCP抗体检测方法的应用及其与临床相关性分析  

Application of Own Established Anti-CCP Antibody Methods and Its Clinical Correlation Analysis

作　　者：房国祥[1] 虞伟[2] 谢云 

机构地区：[1]常州市德安医院,江苏常州213003 [2]南京军区南京总医院解放军检验医学研究所,江苏南京210002 [3]常州市戚墅堰社区卫生服务中心

出　　处：《中国误诊学杂志》2011年第2期264-267,共4页Chinese Journal of Misdiagnostics

摘　　要：目的应用自建的抗CCP抗体等检测方法,用于类风湿性关节炎(RA)患者的的研究,并分析相应的实验指标与临床的相关性。方法分别检测自身免疫性疾病患者的抗CCP抗体、RF、ANA、CRP和ESR等指标,并了解患者的临床表现及影像学资料。结果 RA患者的抗CCP抗体的阳性率、RF及CRP的水平均显著高于各对照组,差异有统计学意义(P<0.05);RA患者中抗CCP抗体阳性者在关节肿痛数、破坏数和关节外症状方面有较阴性组患者严重的趋势,且抗CCP抗体水平与骨侵蚀程度相关。43例RF阴性的RA患者中有8例(18.6%)其抗CCP抗体明显升高。结论检测抗CCP抗体及相应实验指标,是可用于RA等自身免疫性疾病研究的有效方法,可为临床诊断、评价疗效和预后提供一定的实验室参考依据。Objective To apply the established anti-CCP detection methods in order to study the patients with rheu- matoid arthritis. Then to analyse the correlation between experiment index and clinic appearance. Methods Isolating peripheral blood from patients with autoimmune disease was examined the item such as anti-CCP antibody,rheumatold factor（RF） ,antinuclear antibody { ANA） , C-reactive protein （CRP） and erythrosedimentation （ ESR} et al. Clinical situation and imageology information were analysed. Results The postive rate of anti-CCP antibody and the concentration of RF and CRP in RA was significant higher than control group. It had statistical significance[P~ 0.05 ）i The patients of RA with anti-CCP antibody postive had severe tendency than patients with anti-CCP antibody negative in the number of joint with swelled and painful or destroied. It had the game tendency of ex-joint symptom. Anti-CCp antibody serum level were of correlation with the severity degree of bone erosion. The RF concentration of RA patients with anti CCP antibody postive was significant higher than patients with anti-CCP antibody negative. There were 18.6%（8/43）RA patients with RF negative which they had higher anti-CCP antibody serum level. Conclusion To detect anti-CCP antibody serum level and other experiment index are effective methods for the research of RA and other autoimmune disease patients. It will provid message for clinical diagnosis and evaluate therapeutic effect or prognosis.

关 键 词：自身抗体/分析 肽类 环/分析 关节炎 类风湿/诊断 人类 

分 类 号：R593.22[医药卫生—内科学]