红外光谱用于大肠肿瘤诊断的初步研究  

Initial Research on Fourier Transform Infrared Spectroscopy for the Diagnosis of Colon Neoplasms

作　　者：姚宏伟[1] 刘亚奇[1] 傅卫[1] 石雪迎[2] 张元福[3] 徐怡庄[3] 

机构地区：[1]北京大学第三医院普通外科,北京100191 [2]北京大学医学部病理学系,北京100191 [3]北京大学化学与分子工程学院,北京100871

出　　处：《光谱学与光谱分析》2011年第2期297-301,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50673005);北京市优秀人才培养项目(2010D009001000005)资助

摘　　要：探索红外光谱诊断大肠肿瘤性质的可行性。联合使用衰减全反射探头与傅里叶变换红外光谱仪测定新鲜离体大肠18个癌组织和10个良性腺瘤的红外光谱,将两组光谱的峰位及相对峰强等指标进行比较。与良性腺瘤相比,大肠癌的红外光谱具有如下特征:(1)与脂类相关的谱带2 925和1 740cm-1峰强比I2 925/I1 460(p=0.018)和I1 740/I1 460(p=0.009)明显降低,表明癌组织的脂类相对含量降低;(2)与蛋白质相关峰强比值I3 375/I1 460(p=0.012)和I1 550/I1 460(p=0.041)明显升高,表明蛋白质相对含量明显升高;(3)与核酸相关的1 080cm-1处峰位发生明显的蓝移(p=0.039),峰强比I1 080/I1 460明显升高(p=0.036),说明癌组织中核酸相对脂类含量明显增加;(4)谱带1 305cm-1处峰位发生明显的红移(p=0.041),其指认有待进一步研究。初步研究结果表明,傅里叶变换红外光谱分析可能成为一种快速鉴别大肠肿瘤性质的新方法。To explore the feasibility of Fourier transform infrared spectrometry（FTIR） for the diagnosis of colon neoplasms,fresh samples of eighteen cancers and ten adenomas were collected during colon surgery and were measured by FTIR via probe of attenuated total reflection（ATR）.The peak position and the intensity of all bands were measured and compared between the malignant and benign groups.Results show that the FTIR of malignant neoplasm was different from that of benign one.（1）For the bands related to lipid： The ratio of I2 925/I1 460（p=0.018） and I1 740/I1 460（p=0.009） decreased in cancer FTIR spectrum,indicating the lower relative quantity of lipid in malignant neoplasm.（2）For the bands related to protein： The ratios of I3 375/I1 460（p=0.012） and I1 550/I1 460（p=0.041） increased in cancer,which shows that the relative quantity of protein was more in cancer than that in adenoma.（3） For the band related to nucleic acid： In malignant tissue the peak position in 1 080 cm-1 shifted toward a higher wave number（p=0.039）,compared with that in benign one.And the ratio of I1 083/I1 460 increased significantly（p=0.036）,illustrating the higher relative quantity of nucleic acid to lipids in malignancy.（4） The peak position of 1 305 cm-1 shifted to a lower wavenumber（p=0.041）,which should be determined in further research.It is concluded that our initial research showed that the FTIR maybe develops into a promising method for rapid differential diagnosis of colon malignant and benign neoplasms.

关 键 词：傅里叶变换红外光谱 大肠肿瘤 诊断 

分 类 号：O657.3[理学—分析化学]