肾形肾状线虫(Rotylenchulus reniformis)浙江杭州群体形态学和分子特性及寄主范围  

Morphological and molecular characterization and host range of Rotylenchulus reniformis population occurring in Hangzhou,Zhejiang,China

作　　者：张燕[1] MATAFEO Angelika 石红利[1] 郑经武[1] 

机构地区：[1]浙江大学农业与生物技术学院生物技术研究所,杭州310029

出　　处：《植物病理学报》2011年第1期37-43,共7页Acta Phytopathologica Sinica

基　　金：农业部公益性行业科研专项(nyhyzx07-050-6);浙江省科技厅资助项目(2008C22062);浙江省自然科学基金杰出青年团队资助项目(R307396)

摘　　要：肾形肾状线虫（Rotylenchulus reniforrnis）是一种重要的蔬菜线虫，寄主范围和分布很广。本文根据形态学特征和形态测量将来自浙江省杭州市蔬菜上的肾状线虫群体鉴定为肾形肾状线虫，为浙江省首次报道。该群体的形态学特征及形态测量值与模式标本基本吻合，个别形态测量值（比值）有变异。通过对该肾形肾状线虫群体的rDNA中ITS区和编码28SRNA基因中的D2D3区的PCR扩增和序列测定，明确二序列长度分别为808bp和786bp；序列分析发现该群体ITS序列与肾形肾状线虫种内一些两性生殖群体的亲缘关系较近，同源性高达99．5％～100％，而与一孤雌生殖群体同源性为92．4％；编码28SRNA基因中的D2D3区与已报道群体的同源性达到99．4％，相似度较高；本研究还发现杭州肾形肾状线虫群体的寄主涉及11个科16个属的果蔬。Rotylenchulus reniformis is one of the most important vegetable nematodes, and has widely host range and distribution. Based on the morphological and molecular characters, a Rotylenchulus population from Hangzhou, Zhejiang, China, in vegetable crops was identified as R. reniformis. Most morphometrics of Hang- zhou population of R. reniformis were identical with type specimens and some variations were detected. PCR Amplification and sequencing of ITS and D2D3 region of 28S RNA gene found that the two sequence lengths were 808 bp and 786 bp, respectively. Sequence alignment of ITS region of Hangzhou population and other inter-populations of R. reniformis, showed that Hangzhou population had 99.5% to 100% similarity with the three amphimitic populations, whereas 92.4% with that of one parthenogenic populations. The D2D3 region sequence of 28S RNA gene of Hangzhou population had the similarity of 99.4% to that of a R. reniformis populaiton in GenBank. The host range of Hangzhou population of R. reniformis included fruit and vegetable crops in 16 genera of 11 families. This is the first report of the R. reniformis in Zhejiang Province.

关 键 词：肾形肾状线虫 形态学 rDNA特征 寄主范围 

分 类 号：S432.45[农业科学—植物病理学]