利用酵母双杂交研究葡萄卷叶伴随病毒2号p24与寄主互作的初步结果  

Preliminary study on interactions between p24 of Grapevine leafroll-associated virus 2 and the host by yeast two-hybrid screening

作　　者：费菲[1] 刘命华[1] 周涛[2] 范在丰[2] 程玉琴[1] 

机构地区：[1]中国农业大学农学与生物技术学院果树系/北京市果树逆境生理与分子生物学实验室,北京100193 [2]中国农业大学农学与生物技术学院植病系,北京100193

出　　处：《植物病理学报》2011年第1期102-105,共4页Acta Phytopathologica Sinica

基　　金：中央高校基本科研业务费专项资金(2009-2-06)

摘　　要：葡萄卷叶病（Grapevine leafroll disease，GLRD）是一种世界性病毒病害，严重影响葡萄生产和葡萄酒产业。至今已报道11种具不同血清学关系的葡萄卷叶伴随病毒（Grapevine leafroll—associated virus，GLRaV）均可引起葡萄卷叶症状，其中GLRaV-2为长线形病毒属（Closterovirus）成员。In this study, the bait plasmid （pGBK-p24） with the full length of p24 encoded by Grapevine leafroll-associated virus 2 Liaoning isolate （GLRaV-2-LN）, and grapevine cDNA library were constructed, and then grapevine cDNA library was screened by the bait plasmid to identify protein （ s ） that interact with p24. The results showed that the aa sequence and open reading frame of the insert cDNA fragment of p24 were correct, and the bait plasmid has no self-activating effect on yeast strain AH109 and Y187; The cDNA library had a titer of 2.2 × 10^6 cfu/ mL, and most insert fragments were more than 700 bp in size , which indicated that the cDNA library are qualified for the yeast two-hybrid screening. 19 candidate postive clones displayed specific interaction with p24 and 16 cDNA sequences of these clones were obtained. BLAST showed that these cDNAs encode 8 proteins, such as MYC transcription factor, aconitase and type I inositol polyphosphate 5- phosphatase, etc; other three cDNA sequences encoded the same unnamed protein.

关 键 词：葡萄卷叶病 病毒病害 酵母双杂交 伴随 P24 互作 寄主 利用 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]