养肝利胆颗粒对胆固醇结石小鼠肝脏中PPAR-γ及CYP7A1表达的影响  

Effects of YangGanLiDan Granule on PPAR-γ and CYP7A1 of Liver in Mice with Cholesterol Gallstone

作　　者：梁晓强[1] 顾宏刚[2] 章学林[2] 张静喆[2] 刘胜[1] 

机构地区：[1]上海中医药大学附属龙华医院中医外科研究所,上海200032 [2]上海中医药大学附属龙华医院普外科,上海200032

出　　处：《辽宁中医杂志》2011年第1期172-174,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：上海市教委创新团队资助课题;上海市医学领军人才资助课题(LJ06048);国家教育部高等学校博士点基金(200802680005)

摘　　要：目的:观察养肝利胆颗粒对胆固醇结石小鼠肝脏PPAR-γ及CYP7A1表达的影响。方法:38只C57BL/6雌性小鼠随机分为正常对照组(n=10)、模型组(n=15)和养肝利胆颗粒组(n=13)。其中后两组小鼠采用高脂饮食诱发法建立胆固醇结石模型。造模过程中,养肝利胆颗粒组小鼠予养肝利胆颗粒2.1g/(kg.d)灌胃治疗。8周后观察各组小鼠的成石率,并用Western Blotting法检测肝脏中PPAR-γ及CYP7A1的表达。结果:养肝利胆颗粒组成石率显著降低(P<0.01),小鼠肝脏PPARγ及CYP7A1表达增强。结论:养肝利胆颗粒可通过增强肝脏PPARγ及CYP7A1的表达,从而发挥防治胆石病的作用。Objective：To observe the effects of YangGanLiDan granule （YGLD） on liver perosisome proliferator activated receptor gamma （PPAR-γ） and Cholesterol 7 alpha-hydroxylase （CYP7A1） in C57BL/6J mice model with cholesterol gallstone. Methods：Thirty-eight C57BL/6J mice were randomly divided into normal control group （n = 10） ,untreated group （n = 15） and YGLD group （n = 13） . Cholesterol gallstone was induced in mice of the latter two groups by feeding high cholesterol diet. Mice in the YGLD group were intragastricly administered YGLD 2. 1g /（kg·d） . After 8-weeks treatment,animals were sacrificed and sampled to calculate the incidences of stone formation. The expression of PPAR-γ and CYP7A1 in liver tissues were detected. Results：The incidences of stone formation were 73. 33% in untreated group,0% in normal control group,and 30. 77% in the YGLD group. And the incidence in untreated group was significantly higher than those in the normal control group and the YGLD group（P 0. 01） . The expression of PPAR-γ and CYP7A1 were up-regulated after the treatment of YGLD. Conclusion：YGLD can decrease the incidence of stone formation,regulate PPAR-γ and CYP7A1 up,which may be one of the mechanisms in the treatment and prevention of cholesterol gallstone disease.

关 键 词：养肝利胆颗粒 胆固醇结石 小鼠 PPAR-Γ CYP7A1 

分 类 号：R285.4[医药卫生—中药学]