揉髌手法对兔膝关节软骨细胞凋亡基因表达的影响  

The Influence Of The Rou-Bin Manipulation On Knee Joint Chondrocytes Gene Expression Of Apoptosis In Rabbit

作　　者：戴七一[1] 欧国建[1] 崔伟[1] 林光琪[2] 覃学流[1] 刘靖[2] 姚平[1] 黎强[2] 腾居赞[1] 容向宾[1] 辛晓东[1] 袁经阳[1] 

机构地区：[1]广西中医学院附属瑞康医院,广西南宁530011 [2]广西中医学院,广西南宁530001

出　　处：《辽宁中医杂志》2011年第1期174-176,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30860359);广西科技厅自然基金资助项目(桂科自0832178)

摘　　要：目的:观察揉髌手法对兔膝关节软骨细胞凋亡的影响,探讨手法治疗的作用机制。方法:随机将50只新西兰兔分为手法组、药物组、模型组、假模组和正常组,每组10只;前3组手术造成骨内高压型模型,假模组手术但不损伤相应血管,正常组不做任何处理。手法组每只兔揉髌手法治疗17次,药物组膝关节内注射玻璃酸钠注射液5次,其他兔无特殊处理。造模后12周末处理所有动物,取右膝内侧胫骨平台软骨,采用免疫组化法检测软骨细胞凋亡基因Bcl-2、Bax、Fas表达,统计学处理。结果:手法组Bcl-2表达(2.67±0.50),模型组(0.33±0.52)(两组比较P<0.01);手法组Bax(1.30±0.67),模型组(2.44±0.73)(两组比较P<0.01);手法组Fas(1.56±0.73),模型组(2.67±0.52)(两组比较P<0.01)。结论:揉髌手法通过上调Bcl-2,下调Bax、Fas基因表达减少兔膝关节软骨细胞凋亡,可能是延缓兔膝关节软骨组织退变的机制之一。Objective：To observe the Rou-Bin Manipulation on apoptosis of knee joint chondrocytes in rabbit,explore the influ-ence of manipulation mechanisms. Methods：50 New Zealand rabbits devieded randomly into five groups：Manipulation group,drug group,Model group,dummy-model group and the normal group,10 rabbits in each group; the first 3 groups cause IOH-model by operative,dummy-model group operate without damage the corresponding vessel,no treatment for the normal group. 17 times of Rou-Bin manipulation on each rabbit in manipulation group,the rabbit injection with sodium hyaluronate 5 times in drug group,no special treatment for other rabbits. 12 weeks after modeled,to take all the rabbit right knee medial tibial plateau cartilage tissue processing,detected chondrocyte apoptotic gene Bcl-2、Bax、Fas expression by using immunohistochemical method,statistics processing. Results：Bcl-2 expression result（2. 67 ± 0. 50） for manipulation group,（0. 33 ± 0. 52） for model group（P 0. 01 to compare with both groups） ; Bax expression result（1. 30 ± 0. 67） for manipulation group,（2. 44 ± 0. 73） for model group（P 0. 01 to compare with both groups） ; Fas expression result（1. 56 ± 0. 73） for manipulation group,（2. 67 ± 0. 52） for model group（P 0. 01 to compare with both groups） . Conclusion：The Rou-Bin manipulation reduced apoptosis of knee joint chondrocytes in rabbit through up-regulating Bcl-2 expression and down-regulating Bax、Fas expression,possibly one of the mechanism of delay the degeneration of cartilage tissue of knee joint in rabbit.

关 键 词：手法 兔 细胞凋亡 基因表达 实验研究 

分 类 号：R244.1[医药卫生—针灸推拿学]