商陆小G蛋白激活蛋白基因PaAGAP在逆境下表达研究  

Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress

作　　者：刘芳[1] 吴亮其[1] 王辉[1] 张媛雅[2] 柴团耀[1] 

机构地区：[1]中国科学院研究生院,北京100049 [2]中国矿业大学,北京100083

出　　处：《中国科学院研究生院学报》2011年第1期116-124,共9页Journal of the Graduate School of the Chinese Academy of Sciences

基　　金：国家高技术研究发展计划(863)项目(2006AA10Z407;2007AA021404);中国科学院研究生院院长基金B类项目(085102FN00)资助

摘　　要：利用同源克隆和RACE方法在商陆(Phytolacca acinosa)中得到一个推测的小G蛋白ArfGTPase激活蛋白ArfGAP(ArfGTPase activating protein)的全长cDNA序列PaAGAP.PaAGAP序列编码332个氨基酸.蛋白含有保守的锌指结构域(CX2CX16 CX2C类)和C2结构域.实时荧光定量PCR表明在寒、旱、盐和重金属的不同时间胁迫下,PaAGAP可以被诱导,表达量有不同程度的上调.由此推测PaAGAP可能是通过快速启动转录和翻译,使ARF失活,抑制植物生长素极性运输,来参与植物逆境反应的.A full-length cDNA of an ArfGAP（PaAGAP） encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE.PaAGAP is predicted to contain a CX2CX16 CX2C type zinc finger domain and a C2 domain.Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature,osmotic,salt,and heavy metal stresses,indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport.

关 键 词：商陆 ARFGAP 锌指 逆境 表达 

分 类 号：Q943.2[生物学—植物学]